In 5.4 mm single slot chips, sheep blood was flowed at 3 µL/min though the 3 mm x 1 mm channels with PBS flowed at the same rate but in the opposite direction for four hours. The chips were pre-wetted…

Hello Everyone, For the next figure in our paper, and to make the transition happens smoothly from Track Etched membranes to NPN membranes, we decided to run the same beads experiment on NPN membranes as well. So Kilean and i…

As Aim 1 of my thesis involves using theoretical models to be able to predict particle capture, it is important for me to fully understand how each model system behaves when compared to experimental results. One of the most important…

Introduction I am working part time in collaboration with the Schwarz lab (CMSR, University or Rochester) on a bacterial migration project that includes the use of SimPore’s membranes. In order to increase throughput, Aline was contracted out to manufacture multiplex…

Intro/Motivation:   SiR-Hoechst is a far-red DNA stain for live-cell micro/nanoscopy and has been shown to be non-cytotoxic. I mentioned to Tom that if we can label the cells we can better automate the cell tracking for faster analysis. Tom knew of this…

We are working on finishing up a manuscript on our nanosphere lithography work. this post shows some of the figures for said publication. This paper includes work done with 300 and 200 nm NS as preliminary work, and then focuses…

Hello Everyone, Following of making figures for our first exosome paper, I needed to have SEM images after releasing step. So i ran another experiment and checked the membrane after releasing step. You can see the SEM images of this…

Introduction Over the past two decades there has been little to no improvement in treatment for people who have end stage renal disease (ESRD). The two main methods of treatment for ESRD are kidney transplant or dialysis. However, due to…

Hello Everyone, Following up the discussion that we had in my last post https://trace-bmps.org/sem-images-for-micro-nano-scale-experiments/about my SEM images after capturing and cleaning. It was suggested to tilt the samples and make sure that beads are inside the pores.    So I…

Introduction When concerning the issue of adherence on any substrate, the main two interactions that matter most are that of the cell interacting with the basement membrane (whether cell produced or artificially added) or the interaction between the protein based…