So in the past couple of months, I have focused on wrapping up the microdialysis project that was initiated by Alec. Just as a recap, this experiment utilizes a modified SepCon that features an open bottom to allow for separation…
Category: NRG
SEM of Sheared/Non-sheared HUVECs
Motivation and Procedure After discovering that pores on 3um LP SiO2 membranes seem to get blocked following 3 hours of shear [of HUVECs], Kilean and I decided to utilized SEM to further inspect what might be doing the occluding. HUVECs…
Posted in NRG
RTP Ramp Rate Effect on 6W Bias pnc-Si Stack Increases Pores Size Slightly
Purpose: We want to know the effects of the RTP process on pore size, pore density for the biased films coming out of the AJA. I believe that the RTP ramp rate is important for forming bigger pores. Some work (Joe…
Posted in NRG
Rotation Project – Measuring Transmembrane Resistance and Pressure to Observe Membrane Saturation
Hi all, my name is John Cognetti, and I am a first-year PhD student in BME. I recently finished a rotation in the McGrath Lab. My goal was to measure changes in resistance across the membrane as the membrane becomes…
Posted in NRG
Nanovesicle Rigidity: Can Exosomes be Modeled as Rigid Particles?
This an extremely important topic that has come up multiple times in discussions within NRG as well as during my qualifying exam. The question is: are gold nanoparticles a good model for exosomes? The reason that this question comes up…
Posted in NRG
moving average angle autocorrelation and wound healing analysis
Traditional Wound Healing Analysis (the implicit gold standard) Wound recovery is reported base on the closing of the two wound edges. Proposed Wound Healing Analysis (cell counting/tracking methods): Wound recovery is reported base on the migration/movement of the wound…
Posted in NRG
Cellular Adhesion: Bare NPN v PEG NPN
In 5.4 mm single slot chips, sheep blood was flowed at 3 µL/min though the 3 mm x 1 mm channels with PBS flowed at the same rate but in the opposite direction for four hours. The chips were pre-wetted…
Posted in NRG
Beads experiments with NPN membranes
Hello Everyone, For the next figure in our paper, and to make the transition happens smoothly from Track Etched membranes to NPN membranes, we decided to run the same beads experiment on NPN membranes as well. So Kilean and i…
Posted in NRG
Transmembrane Pressure Monitoring: Track Etch vs. NPN
As Aim 1 of my thesis involves using theoretical models to be able to predict particle capture, it is important for me to fully understand how each model system behaves when compared to experimental results. One of the most important…
Posted in NRG
Aline Devices for Cell Culture
Introduction I am working part time in collaboration with the Schwarz lab (CMSR, University or Rochester) on a bacterial migration project that includes the use of SimPore’s membranes. In order to increase throughput, Aline was contracted out to manufacture multiplex…
Posted in NRG
