Introduction/Motivation A problem many of us face in day to day research is the ability to change membrane format in a days to weeks notice. The cost of developing an entire new wafer of membranes for preliminary experiments is costly…

Hi all, recently we improved cell tracking with the use of SiR-DNA label (see highlights below).   random migration:  A. Representative cell tracking from three independent migration experiments, color-coded by the color bars to the left in red, green, and…

In the past, I have been working on developing mechanisms to inhibit neutrophil migration through endothelial basement membrane. Please find the previous blogs on the following links https://trace-bmps.org/updates-on-neutrophil-collagen-migration-experiments/ https://trace-bmps.org/manipulating-neutrophil-migration-through-the-vascular-barriers/ https://trace-bmps.org/sem-imaging-of-neutrophil-transmigration-through-collagen-gel/   This is a small update on the following. CD29…

Figure 2 – Micro scale Experiments with 10 micron fluorescent particles and 8 micron pore size track etched membranes: A) Fluorescent intensity – time plot showing increasing in the intensity signal during the capturing step and decreasing for releasing step.…

So in the past couple of months, I have focused on wrapping up the microdialysis project that was initiated by Alec. Just as a recap, this experiment utilizes a modified SepCon that features an open bottom to allow for separation…

Motivation and Procedure After discovering that pores on 3um LP SiO2 membranes seem to get blocked following 3 hours of shear [of HUVECs], Kilean and I  decided to utilized SEM to further inspect what might be doing the occluding. HUVECs…

Purpose: We want to know the effects of the RTP process on pore size, pore density for the biased films coming out of the AJA. I believe that the RTP ramp rate is important for forming bigger pores. Some work (Joe…

Hi all, my name is John Cognetti, and I am a first-year PhD student in BME. I recently finished a rotation in the McGrath Lab. My goal was to measure changes in resistance across the membrane as the membrane becomes…

This an extremely important topic that has come up multiple times in discussions within NRG as well as during my qualifying exam. The question is: are gold nanoparticles a good model for exosomes? The reason that this question comes up…

Traditional Wound Healing Analysis (the implicit gold standard) Wound recovery is reported base on the closing of the two wound edges.   Proposed Wound Healing Analysis (cell counting/tracking methods): Wound recovery is reported base on the migration/movement of the wound…