Category: NRG

Study Proposal:In Vitro Cell Adhesion: Whole Blood, Single channel 5.4 mm chips

The purpose of this study will be to compare the cellular adhesion on PEG vs Bare nano porous silicon nitride membranes using the same fluidic devices as the in vitro clearance study. The devices will need to be disassembled for…

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Kidney Week 2017

I recently attended ASN’s Kidney Week 2017 in New Orleans. I mostly ate beans and rice, although there are three completely vegan restaurants and several restaurants with vegan options. The music was terrific and the weather was terrific, the people…

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SEM imaging of neutrophil transmigration through collagen gel

Kilean re-imaged some of my old samples with neutrophil transmigrating through the collagen gel. This sample was prepared and imaged on June 25th, 2017, and kept under dry conditions. Surprisingly, the top gel kinda got delaminated and we can see…

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Dialysis Pressure Balancing

Background For the in vitro portion of our dialysis project here at UR, we have settled on using two major experiments to test our devices for eventual transition to an in vivo model. These experiments include a single pass system…

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Stability of 200nm SiO2 + 20nm SiN chips

The plan was to fabricate ~7nm pores on the PDMS-painted 200nm SiO2 + 20nm SiN chips and take IVs overtime, and observe the stability of the nanopore’s size. I fabricated a total of 8 200nm SiO2+20nm SiN chips using the…

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Henry’s BMES 2017 highlights

[NOTES] BMES 2017 highhlights

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BMES 2017

Here is a summary of some of the talks I attended at BMES 2017, mainly from the Nano and Micro- technologies track. This is an ongoing post, I will post more comments, links, and figures. Anna Kalmykov (Tzahi Cohen-Karni) Carnegie…

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Exosome Scale Experiment (Summary)

Hello Everyone, So following up on my exosome scale experiment and my last post, i am presenting my last update to summarize my results and to show that my results are trustable and reproducible. This is how we take images:…

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NanoParticle Tracking Analysis

Hello Everyone, Kilean helped us to set a training session for NanoParticle Tracking Analysis (NTA) in UR Medical Center. For this training session, i had 4 samples including: Beads, Liposomes, Media + 10% serum, and PBS. Here  is a summary…

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Monolith X-sections

As the title states, these are images from the monolithically fabricated devices used by Kyle and Vincent in Ottawa. These devices were fractured with tweezers, then tilted and metallized with 5 nm of Pt, mounted on carbon tape.    …

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