I’ve now made 18 pores in monolith devices, with only 1 real success, which was the first one that I report on already. All of the rest have failed for various reasons, mainly relating to the silicon nitride membrane in…

The plot below shows a calibration curve to calculate the etching time needed during the RIE step (used to reduce the size of the nanospheres) to obtain a desired pore size. The membranes were patterned from nanospheres with a nominal…

As the title states, I have been making TEM images and reconstructions from a variety of NPN materials. I hope to correlate the underlying nanostructure with SERS activity eventually. These images show many different porosities, topographies and pore shapes. Wafer…

So Alec, Tejas and I will have a poster at the Immune Imaging Symposium in the Med Center on Nov. 4th and we created an image for our abstract that Jim requested I post here in higher quality. Immune Imaging…

Last week, I attended the American Society of Exosomes and Microvesicles (ASEMV) conference in Monterey, Ca and presented a poster on the work that I’ve been doing. Despite a very low turnout for my poster session (due to the scheduling…

Hi all, This is a “take two” of the mock figure for our manuscript titled “Porous substrates influence early endothelial migration and the associated fibronectin fibrillogenesis”. Many new figures are produced based on the reviewer suggested experiments. It is likely…

For last many months, I am working on neutrophil migration experiments to develop protocols to inhibit them from traversing the basement membrane. My previous blogs are here and here. I want to prevent neutrophils from crossing the endothelial basement membrane…

My goal and focus of the last post is to to use the existing SU-8 grid structure used on the ultrathin silicon oxide lift off membranes to create . To accomplish the goal the SU-8 surface needs to be modied…

This post offers an update on membrane fabrication through nanosphere lithography (NSL), describing in more detail the releasing step as well as the membrane integration into PDMS devices. Membrane lift-off is achieved by dissolving a ZnO film underneath the membrane.…

So this is basically an update of my post from a couple of weeks ago (and a figure from the poster that I just put up), but it is a very good status update of the contamination experiments that I…