One of the major goals of my work with capturing exosomes is to show that our purified population is less contaminated with protein than exosomes purified by ultracentrifugation. This is important because the protein (and all the other junk floating…

Some of my recent cell culture work on Microporous MgF2 windows have shown vacancies in the cultured monolayer after fixation and staining. We should see a contiguous layer. To better understand my fixation, I seeded and cultured P10 RPE-19 cells…

Introduction Endothelial cell detachment under flow on porous substrates has been a concern in our sepsis research since I began my work in the lab, and some time before that as well. As we plan on moving to microfluidic models…

This post includes some optical and SEM micrographs of a SiO2 membrane patterned by nansosphere lithography using 300 nm polystyrene beads. An SU-8 microgrid was deposited on top and the membrane was released by dissolving a bottom layer of ZnO.…

I am attempting to manufacture a range of npMgF2 thicknesses as demonstrated in my recent CCD post. Yesterday, I undershot my thicknesses substantially, I believe because of construction vibrations in the building. However, this results in some of the thinnest…

In my previous blog, I alluded to the issues I was facing in achieving successful migration of neutrophils through the basement membrane into the collagen gels. I am successfully able to achieve transendothelial migration, but migration beyond the endothelial basement…

I have three monolith experiments to report, one of which was successful. In all three cases, I was able to make a pore, though it took about 5 times longer than it would have for the NPN-tented chips. This is…

Now that we have a number of ways to quantify the nanostructure of different npMgF2/npAu film stacks, we can begin an optimization experiment for which combination of factors will be useful to us. To explore the space of outcomes, we…