Category: NRG

1255 (100nm NPSN) Partially Etched Super Thin TEMs

I’ve been trying to recover a pore distribution from my 1085 MgF2 SERS wafer that had a ~60 nm pore size on average. This has been difficult with thinner, 50 nm NPN because there is less material to shape; it’s

Posted in NRG

Adenovirus Captured and Imaged

Last week we were able to capture and image individual virions on the nano membrane without a PETL using a simple drop-on-membrane protocol to establish some pull through. This was done to to verify that the individual virions will actually

Posted in NRG

Microporous MgF2 Cell Seeding Density over 3 days

One of the issues with my microporous MgF2 cell culture experiments is the apparent multilayer of cells that form on the freestanding area; ideally we would have a nice cell monolayer for imaging. It has been unclear if this was

Posted in NRG

700nm~1um thickness photoresist gasket

There are four main challenges for making such a thin photoresist gasket on a little chip. Q1: How can I get under 1um thickness photoresist gasket? Q2: Is the adhesion of photoresist gasket strong enough for further usage? Q3: How

Posted in NRG

Issues in achieving Neutrophil migration through collagen gels

For many months, Emma and I are trying to achieve neutrophil migration through endothelial cells and through the underlying collagen gel. I need to explain the details of my device first. The schematic is shown as below. I am using

Posted in NRG

ICOM 2017

Grand Ballroom A Chair: Baoxia Mi & Zhiping Lai 09:00-09:25 [O8.01] Synthesis of graphene oxide membranes and their behavior in water and isopropanol A. Aher*1, Y. Cai1, M. Majumder2, D. Bhattacharyya1 1University of Kentucky, USA, 2Monash University, Austria 09:25-09:50 [O8.02] Carbon

Posted in NRG

Folding Suppression Experiments

A quick recap: of the pores using the old NPN batch, 2 of them showed folding suppression. The first one I was able to collect events for 1000 and 2000 bp dsDNA, bnoth of which showed almost no folding, but

Posted in NRG Tagged with:

Residue on SiO2 membranes is dependent on wafer lot, but not storage location

Last meeting, we discussed the possibility of the storage gel-boxes outgassing a residue onto the porous SiO2 membranes, as evidenced by cells not adhering to the membranes and difficulty in acquiring SEM images. Possible Gel-Box Outgassing on Porous SiO2 Membranes

Posted in NRG

Neutrophil Transmigration through Collagen Gels

Intro For the past couple months, I have been working with Tejas to recreate his previous experiments using a more complex system. The goal of the research is to study the transmigration of neutrophils and represent how they behave during

Posted in NRG

Immunogold Labeling of Exosomes

So, this is the data that we have all been waiting for. Without all the fancy introductions and stuff, I present you with labeled exosomes. They were isolated in tangential flow from my human plasma samples using the SOP that

Posted in NRG
0
    0
    Your Cart
    Your cart is emptyReturn to Shop