Category: NRG

Ultrathin ~10nm npMgF2 TEM structure

I am attempting to manufacture a range of npMgF2 thicknesses as demonstrated in my recent CCD post. Yesterday, I undershot my thicknesses substantially, I believe because of construction vibrations in the building. However, this results in some of the thinnest…

Posted in NRG

Updates on neutrophil-collagen migration experiments

In my previous blog, I alluded to the issues I was facing in achieving successful migration of neutrophils through the basement membrane into the collagen gels. I am successfully able to achieve transendothelial migration, but migration beyond the endothelial basement…

Posted in NRG

First experiments with monoliths

I have three monolith experiments to report, one of which was successful. In all three cases, I was able to make a pore, though it took about 5 times longer than it would have for the NPN-tented chips. This is…

Posted in NRG

Central Composite Design for Optimizing SERS Substrates

Now that we have a number of ways to quantify the nanostructure of different npMgF2/npAu film stacks, we can begin an optimization experiment for which combination of factors will be useful to us. To explore the space of outcomes, we…

Posted in NRG

1255 (100nm NPSN) Partially Etched Super Thin TEMs

I’ve been trying to recover a pore distribution from my 1085 MgF2 SERS wafer that had a ~60 nm pore size on average. This has been difficult with thinner, 50 nm NPN because there is less material to shape; it’s…

Posted in NRG

Adenovirus Captured and Imaged

Last week we were able to capture and image individual virions on the nano membrane without a PETL using a simple drop-on-membrane protocol to establish some pull through. This was done to to verify that the individual virions will actually…

Posted in NRG

Microporous MgF2 Cell Seeding Density over 3 days

One of the issues with my microporous MgF2 cell culture experiments is the apparent multilayer of cells that form on the freestanding area; ideally we would have a nice cell monolayer for imaging. It has been unclear if this was…

Posted in NRG

700nm~1um thickness photoresist gasket

There are four main challenges for making such a thin photoresist gasket on a little chip. Q1: How can I get under 1um thickness photoresist gasket? Q2: Is the adhesion of photoresist gasket strong enough for further usage? Q3: How…

Posted in NRG

Issues in achieving Neutrophil migration through collagen gels

For many months, Emma and I are trying to achieve neutrophil migration through endothelial cells and through the underlying collagen gel. I need to explain the details of my device first. The schematic is shown as below. I am using…

Posted in NRG

ICOM 2017

Grand Ballroom A Chair: Baoxia Mi & Zhiping Lai 09:00-09:25 [O8.01] Synthesis of graphene oxide membranes and their behavior in water and isopropanol A. Aher*1, Y. Cai1, M. Majumder2, D. Bhattacharyya1 1University of Kentucky, USA, 2Monash University, Austria 09:25-09:50 [O8.02] Carbon…

Posted in NRG
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