Last meeting, we discussed the possibility of the storage gel-boxes outgassing a residue onto the porous SiO2 membranes, as evidenced by cells not adhering to the membranes and difficulty in acquiring SEM images. Possible Gel-Box Outgassing on Porous SiO2 Membranes…

Intro For the past couple months, I have been working with Tejas to recreate his previous experiments using a more complex system. The goal of the research is to study the transmigration of neutrophils and represent how they behave during…

Hello Everyone, So as you probably remember, we were able to show the capturing and releasing successfully.   In addition, we have a Matlab Code now which helps us for intensity change, so as you can see in the next…

For the past few weeks, Henry and I have been working with human dermal fibroblast cells and 3T3 mice fibroblast cells. We have been working to quantify wound recovery in hopes to be able to incorporate exosomes from different cell…

Thanks to UR cleanroom and Jim Mitchell, we finally have a better way to deposit the ZnO film that our fabrication process uses as sacrificial layer. Below is a quick comparison of the previous ZnO deposited by sol-gel and the…

Summary Surface roughness is believed to impact the SERS effect. Rougher surfaces tend to produce more SERS activity. We can manipulate the npMgF2 surface by changing the deposition temperature, in the form of changing the grain nucleation, shifting the overall…

Hello Everyone, This is another quick update over our experiments. Exosome Scale Experiments:  In all of our experiments, beads are 100nm and pores are 80nm. As you probably remember, in my last post, i talked about hos the membranes are…

Summary My analysis of npMgF2 has relied heavily on both tomogram and segmentation processes. The tomogram generation has thus far been sufficient, giving enough detail to our eye to identify different structures, but turning the tomograms into 3d models has…