As I’m approaching the end of my time here at UofR, there’s one last thing I’d like to fit into my thesis: experimental validation of the ECMO model. In this post I’m going to describe the experimental design, and in…
Category: NRG
The Need For SEPARATE Metal Films on the Front and Back of NPN Chips
It has been a constant theme in our EO efforts as well as our energy harvesting efforts that our electrodes need to be as close as possible to the surface to see an effect. In Jess’s early EO work, we…
Posted in NRG
More Negative Data for the Nanofluidic Transistor
I don’t think I ever posted it on the blog, but two years ago I discovered something neat. At the time, I was trying to make my nanofluidic transistor, and I was reading Marina’s thesis. She tried to improve the…
Posted in NRG
Ultrasound Generation via NPN Still Doesn’t Work, Even in Low Salt
Two years ago (blog post), I asked Professor McAleavey to help me see if we could use NPN chips to generate ultrasound. I’d found a paper (An exact solution of AC electro-kinetic-driven flow in a circular micro-channel) that suggested, given the…
Posted in NRG
Proof of Concept for a AC Electro-Osmotic Mixer on an NPN chip
I’ve been trying to make the following device work for the past few years: The people who have been studying this have been trying to use the phenomena to mix solutions, or using arrays of mismatched electrodes to generate a…
Posted in NRG
Contact area dictates early cell-substrate interaction for endothelial cells on porous membranes
Abstract Endothelial cell attachment to porous substrates via focal adhesions is initially affected by pore spacing and not absolute porosity. Maturation of endothelial monolayers via cell-cell interactions occurred over the same time course regardless of substrate porosity, pore size and contact area. Cell-matrix interactions at…
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Frequency modeling of Ussing chamber (standard) and iBidi chamber
We desire to test the electrical perfomance of our membranes in the iBidi chamber. For comparison, I am using a gold standard Ussing chamber format to examine the contribution of our membranes to the overall frequency response of a system. I am…
Posted in NRG
Neutrophil-endothelial transmigration through ECM gel scaffolded on microporous silicon nitride membrane
I have been working on developing a microvessel mimetic using our silicon membranes. Just as a recap of vascular biology, the basic components of a microvessel are endothelial cells, the basement membrane secreted by them (presence of perciytes is a…
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Journal Club – Oo et al, 2016 – A nanoporous gold membrane for sensing applications
Paper: Oo2016 Supplementary Info:Oo2016SI Abstract: Design and fabrication of three-dimensionally structured, gold membranes containing hexagonally close-packed microcavities with nanopores in the base, are described. Our aim is to create a nanoporous structure with localized enhancement of the fluorescence or Raman scattering…
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Dialysis Clearance GUI
I have just completed testing and documentation of a MATLAB GUI designed to allow anyone to make predictions of the clearance behavior of solutes though our membranes in tangential flow. I’m uploading the file here, which includes all the MATLAB…
Posted in NRG
