This paper parallels my efforts in creating a microfluidic device that can perform co-culturing of different cell types, allow real time phase and fluorescence imaging, and perform non-invasive TEER measurement using transparent electrodes. This is almost exactly what we aimed…

An application of nanothin and highly porous (NTHP) membranes for mesenchymal stem cell (MSC) differentiation in a co-culture setting. The highlight of this article is that the NTHP membrane allows direct contact of the MSC to cardial myoblast (H9C2) while…

Introduction Permeable membranes provide a physiologically relevant cell culture substrate for in vitro study of barrier cell types, such as endothelial cells, which assume a polarized morphology with distinct apical and basolateral surfaces. Cell morphology and subsequent monolayer formation and barrier…

Here are the Vascular Interviews in comma separated value form if you want to download them. Here is what I learned or had reinforced. An arteriovenous fistula (AVF) is the preferred method for creating an easily cannulated access point to large volumetric blood flow…

A change in open-circuit potential, measured across a pnc-Si membrane, in response to a fluctuation in flow rate of electrolyte solution exhibits a promising flow-sensing capability of the membrane.  To demonstrate this correlation, the open-circuit potentials were measured under a…

I am trying to grow endothelial cells under flow and measure electrical resistance of the culture during the flow-based growth. I am using a peristaltic pump to generate the flow. I am using microporous membrane with 3 micron pores with…

My last experiment did not go as planned, but the results are very interesting nevertheless, and might provide another interesting application for these tw0-membrane systems that we had not yet considered. The start of the experiment went as planned: painted…

I made a size ladder of ALD-ed chips. The chips I began with were ~35 nm average pore size, and I applied 2, 5, 7, 10, and 12 nm of alumina using the ALD in UR nano. This does represent…