Category: NRG

HD Membrane Stability tests: 4 hour Water and PBS

This post is from Xuan ‘Sabrina’ Pan Purpose The nanomembrane used in my experiments is expected to promote efficiency of hemodialysis (HD) for end-stage renal disease patients. The membranes, however, broke during HD for rats, and breaking membranes can lead…

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Energy Harvesting

   

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Liftoff Membrane Capture for Hemodialysis

As part of our work on the NSF STTR grant for our hemodialysis device, we need to develop a device to incorporate our liftoff membranes into to facilitate the fluidics. In this post (and likely, in another post later this…

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Gold sieving graph with all my data and model lines

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Paper review- A versatile lab-on-a-chip tool for modeling biological barriers

This paper parallels my efforts in creating a microfluidic device that can perform co-culturing of different cell types, allow real time phase and fluorescence imaging, and perform non-invasive TEER measurement using transparent electrodes. This is almost exactly what we aimed…

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Journal Club: Nanothin Coculture Membranes with Tunable Pore Architecture and Thermoresponsive Functionality for Transfer-Printable Stem Cell-Derived Cardiac Sheets

An application of nanothin and highly porous (NTHP) membranes for mesenchymal stem cell (MSC) differentiation in a co-culture setting. The highlight of this article is that the NTHP membrane allows direct contact of the MSC to cardial myoblast (H9C2) while…

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Visualization of Endothelial Focal Adhesion Formation on Transparent Porous SiO2 Membranes

Introduction Permeable membranes provide a physiologically relevant cell culture substrate for in vitro study of barrier cell types, such as endothelial cells, which assume a polarized morphology with distinct apical and basolateral surfaces. Cell morphology and subsequent monolayer formation and barrier…

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Protein Adsorption on silanized NPN Chip

We are working with the Shestopalov group to silanize the nanoporous silicon nitride (NPN) membrane chip. In an ideal scenario, the silane would then be conjugated to PEG to ward off protein adsorption. In the presented work, we  study protein adsorption…

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Hemodialysis Vascular Access: Taking the Next Steps (Notes)

Here are the Vascular Interviews in comma separated value form if you want to download them. Here is what I learned or had reinforced. An arteriovenous fistula (AVF) is the preferred method for creating an easily cannulated access point to large volumetric blood flow…

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Cleaning MgF2 Nanomembranes after International Shipping

I had previously manufactured some freestanding MgF2 nanomembranes (50 nm MgF2, starting from Wafer Template 1174) for my work here in Nottingham. I shipped about 75 chips at the beginning of July in my 3d printed holders, however, they arrived with…

Posted in NRG, Protocols
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