Adding pockets (250 micron long, 76 micron deep) to the bottom of the channel (channel is 500 microns wide, 125 deep) in a 4 layer PETL (1 inch long chip) promotes disruption of laminar flow.
Category: NRG
Chip Design for Hemodialysis and ECMO
Recently, SiMPore has been working on a new chip design which involves incomplete etches on both sides in order to create a fluidic system built directly into the silicon, consisting of many long, shallow trenches. These trench chips are designed…
Posted in NRG
Sepsis Retreat
9:10 – 9:55am Tony Pietropaoli Must drink liters of water but not urinating. Retaining. Massive edema. Cases that recover must absorb 1/3 to 1/2 of all patient deaths in hospital are related to sepsis. May be an…
Posted in NRG
Effect of Membrane Morphology on System Capacity During Normal Flow Microfiltration (review)
pdf of Zydney Paper This paper tackles the concept of membrane capacity – the amount of fluid a membrane can pass before it’s flux declines (due to pore blockage or the buildup of a cake) to some arbitrary fraction of…
Posted in NRG
HUVEC alignment on SiN membrane under flow
Hi all, A while back I have posted about my attempt to culture bEnd3 (mouse brain endothelial cell) underflow, which failed to maintain bEnd3 proliferation and did not promote alignment under flow. As an alternative/control, I switch the cell types…
Posted in NRG
Au Nanoparticles deposited on tented posts
Continuing my work from last week, Tucker used the same tenting (NPN, vapor transfer) and incubation procedure (20 nm and 60 nm Au particles) on an oxide substrate with posts (200 nm high). The substrate was dessicated in the 70C…
Posted in NRG
Ozone-treated PDMS: Contact Angles
As seen in prior NRG post, ozone treated samples recover over about 24 hours. However, recent experiments at Gaborski Lab show high variability when using the Corona wand to treat samples with ozone. The goal of this experiment was to…
Posted in NRG
MgF2 Cytocompatibility #4
Summary: I have some ok images (20x) of cells growing on MgF2. My controls still look poor. Something about the stain is causing the TCP + NPN controls to detach ahead of the MgF2 material, even if they aren’t fully dead…
Au Nanoparticles deposited on tented structures
To test the filtration capabilities of our tents, Tucker and I immersed a tented (wafer 1150, ~30 nm pores) oxide structure (~3 um hole diameter, 200 nm high) and a nontented structure in a solution containing 60 nm (.043 nM) and…
Posted in NRG
A Practical Guide to Membrane Transfer
We’ve made a few academic inquiries so far into the theory which governs our nanoporous membrane transfer, but engineers as we are, the pace of our practical knowledge has outstripped that of our theoretical understanding. Here, I want to share…
Posted in NRG
