Hi all, A while back I have posted about my attempt to culture bEnd3 (mouse brain endothelial cell) underflow, which failed to maintain bEnd3 proliferation and did not promote alignment under flow. As an alternative/control, I switch the cell types…

Continuing my work from last week, Tucker used the same tenting (NPN, vapor transfer) and incubation procedure (20 nm and 60 nm Au particles) on an oxide substrate with posts (200 nm high). The substrate was dessicated in the 70C…

As seen in prior NRG post, ozone treated samples recover over about 24 hours. However, recent experiments at Gaborski Lab show high variability when using the Corona wand to treat samples with ozone.  The goal of this experiment was to…

To test the filtration capabilities of our tents, Tucker and I immersed a tented (wafer 1150, ~30 nm pores) oxide structure (~3 um hole diameter, 200 nm high) and a nontented structure in a solution containing 60 nm (.043 nM) and…

We’ve made a few academic inquiries so far into the theory which governs our nanoporous membrane transfer, but engineers as we are, the pace of our practical knowledge has outstripped that of our theoretical understanding. Here, I want to share…

Background: Similar to the Gaborski lab, the authors of the publication “Microfabrication of high-resolution porous membranes for cell culture” fabricated and characterized ultra-thin polymeric membranes for use as cell culture substrates. Upon reviewing their publication, Tom and I discussed some of…

This post is a followup to my previous post, which can be found here. When last I posted, I shared a handful of videos showing membranes transferred onto glass with water vapor being wetted in shear, and noted how the…

Jirachi has been working to make thin nafion films using our membranes as a support structure. Nafion is polymer that contains a sulfonate group, and as a result acts as a proton wire, allowing for cations (+) to be transported across a…

Hi all, a quick update in case you want to know what we are up too.   This summer it will be just me at the McGrath lab; the main goal is to produce some preliminary data with an eye…