Summary The CEIS project ended in June, and I am summarizing the last few weeks of activities below by Josh and me. We did a little more work on reducing the SU-8 film stress through the processing conditions. We also…
Category: NRG
CVB 2015
Name Place Email Description of Interests Status of Discussion Pierre Olivier, organizer Paris pierre-olivier.couraud@inserm.fr No contact Nicola Marchi (Luca’s friend) Montpellier, France Nicola.Marchi@igf.cnrs.fr No contact Lester Drewes (Organizing committee) U Minn (organizing committee ldrewes@d.umn.edu exosome islation from plasma No contact…
Posted in NRG
Control DNA Experiments
A quick update since we last talked about how DNA controls were proving to be problematic: Things are working again (more or less). I went back top basics, remade most of my solution, ordered new DNA samples, and, most importantly,…
Posted in NRG
Vinculin and Focal Adhesion Experiments on TCP
Purpose: To investigate the formation of focal adhesions (FAs) on various cell culture substrates, including TCP, SiO2 membranes, and PDMS membranes. Background: FAs are large macromolecular assemblies through which mechanical force and regulatory signals are transmitted between the extracellular matrix (ECM) and…
Posted in NRG
Nafion-coated SiN Salt Water Filtrations (Part II)
After all the nafion-coated pnc-Si chips that Jirachai gave me broke during my last attempt to filter salt water, Jirachai was kind enough to supply me with a new batch of chips, this one a mix of SiN and pnc-Si,…
PETL mixing with a membrane (not there yet)
I followed Greg’s example and embedded a broken-membrane chip in a PETL. The idea was to use it as a negative control before I use an intact chip. Laminar flow should be maintained in this scenario in order for us…
Posted in NRG
Spreading of HUVECs on Dimpled Sylgard-184 Substrates
To analyze the dimpled Sylgard substrates that we’ve been making, we’ve been conducting experiments to investigate how topographical differences in the surface of a substrate impacts HUVECs and ADSCs, in this case the dimples. We’ve already shown that cells will grow on these membranes in…
Posted in NRG
PDMS Posts
As we were conducting our experiments on dimpled Sylgard substrates we knew that another micro-topography that we wanted to investigate was micro-pillars. In like manner to the dimples, a post microtopography should provide some sort of surface for the…
SEM of geltrex coating on SiN membrane
Hi all, A while back we have shown that geltrex coating enhances the attachment of HUVEC on SiN membrane . Jim believes that geltrex may be extended an “all-purpose” coating to promote the adhesion of different cell types to the SiN…
Posted in NRG
Microporous Membrane Transfer
We’ve had quite a bit of success transferring nanoporous membranes now, but a lot of questions about the exact nature of the physics at work during the transfer process remain. Pursuant to those questions, we wanted to try changing a variable…
Posted in NRG
