Summary: Annealing to above glass transition T (200 C) doesn’t reduce film stress. Adjusting post-exposure bake protocol seems to reduce it. A short post to document my efforts at reducing tensile stress in SU-8-3000 coatings by annealing in vacuum to…
Category: NRG
Burst Pressure Tests: Rectangular Windows
Simpore produced a wafer of 50 nm NPN with a matrix of (4) widths X (5) lengths Widths of 100 um, 300 um, 500 um, and 700 um Lengths of 1 mm, 1.5 mm, 2 mm, 2.5 mm and 3 mm…
Figure drafts for upcoming separations/fouling paper
Figure 1. A) A TEM image of a SiN nanomembrane. Circles with a rim are pores that span the thickness of the membrane, while grey patches are pits in the surface. B) A pore size histogram generated using image processing…
Posted in NRG
Summary of COMSOL simulations for Endohm TEER measurements
This is the final post (hopefully) summarizing my simulation results I did to model Endohm system. Barrett used endohm cup system to do the coculture of bEnd3 and glial cells for his BBB studies. The resistance values obtained while using pncSi…
Posted in NRG
Breathing delaminates nanomembrane tents
I have been having problems getting smoothed sheets of nanomembrane on my little oxide structures. I tried a number of different ways to delaminate the membrane, using tweezers to poke the membrane especially, but had little success getting smoothed sheets.…
Posted in NRG
New Alt. Membrane Release Recipe (Nitride RIE)
I have lost many chips during my MgF2 or Au or Pt process due to yield problems with my RIE. I have wondered if the problem was due to power spikes. I have observed that my most poorly yielding etch…
bEnd3 culture under flow for BBB mimetic (sort of)
Hi all, For the past one month I have been working on a height-based device for culturing bEnd3 (mouse brain endothelial cells) under flow. Ideally, this device is to constitute a blood brain barrier (BBB) mimetic. Device Full View…
Posted in NRG
Cell Culture with the CO2 nanomembrane Devices
Cell Culture on the CO2 exchange Devices The system becomes more complicated with the addition of cells. Tejas Khire cultured HUVECS in a device that had an inverted design to the one pictured above. (The cells were plated on the top portion…
Posted in NRG
MgF2 cytocompatibility #3
I have been trying to verify that my MgF2 material is cytocompatible (e.g. no different than tissue culture plastic), and attempted Live/Dead staining to confirm. The following images are from P6 HUVECs with Invitrogen L3224 Live/Dead stain on MgF2, NPN,…
Posted in NRG
Nanoporous tent wetting fluorescence inconclusive
Due to blog troubles yesterday, we have chosen to upload this post as a .pdf: Nanoporoustentwettingfluorescenceinconclusive
Posted in NRG
