I have been having problems getting smoothed sheets of nanomembrane on my little oxide structures. I tried a number of different ways to delaminate the membrane, using tweezers to poke the membrane especially, but had little success getting smoothed sheets.…
Category: NRG
New Alt. Membrane Release Recipe (Nitride RIE)
I have lost many chips during my MgF2 or Au or Pt process due to yield problems with my RIE. I have wondered if the problem was due to power spikes. I have observed that my most poorly yielding etch…
bEnd3 culture under flow for BBB mimetic (sort of)
Hi all, For the past one month I have been working on a height-based device for culturing bEnd3 (mouse brain endothelial cells) under flow. Ideally, this device is to constitute a blood brain barrier (BBB) mimetic. Device Full View…
Posted in NRG
Cell Culture with the CO2 nanomembrane Devices
Cell Culture on the CO2 exchange Devices The system becomes more complicated with the addition of cells. Tejas Khire cultured HUVECS in a device that had an inverted design to the one pictured above. (The cells were plated on the top portion…
Posted in NRG
MgF2 cytocompatibility #3
I have been trying to verify that my MgF2 material is cytocompatible (e.g. no different than tissue culture plastic), and attempted Live/Dead staining to confirm. The following images are from P6 HUVECs with Invitrogen L3224 Live/Dead stain on MgF2, NPN,…
Posted in NRG
Nanoporous tent wetting fluorescence inconclusive
Due to blog troubles yesterday, we have chosen to upload this post as a .pdf: Nanoporoustentwettingfluorescenceinconclusive
Posted in NRG
Vapor Condensation Inside a Nanocavity
My recent work has focused on wetting the inner portion of an attocavity with isobaric cooling to create condensation. We are trying to attack the problem of wetting through the nanomembrane without breaking the membrane. A perfectly dry membrane will always…
Posted in NRG
Analysis of Barrett’s old TEER data
This blog is to revisit the old TEER data obtained by Barrett from the coculture experiments on pncSi and polymer membranes. The reason behind this analysis is to understand the differences of the raw resistance values obtained for the same…
Posted in NRG
Wetting of Nanocavities using Isobaric Cooling
Summary and Instructional video The simplest way to get something wet is to allow water to condense on the surface of the something. In our case, we have a number of nanocavities (~ 0.1 femtoliters) with porous tents over them. The…
Smooth and Suspended Nanomembrane Tents
I wrote about my experiences tenting nanomembranes here. I delaminated NPN material across a bunch of substrates and found that the film would actually form a nanocavity if spread across a sufficiently small surface (I estimated it to be 1-2…
Posted in NRG
