After the disaster with the fridge freezing our gold nanoparticles and causing them to irreversibly aggregate, I figured that it would be a shame to waste the ruined gold solutions and so I took some conductivity and pH measurements with…

This is a summary I wrote ~3 weeks ago of the work Josh Miller and I are doing under the CEIS grant for NPN Lift-off. Summary: Josh and I have been working for about four weeks so far on this…

You may recall that last year I spent some time working on a proof-of-concept experiment for a high-throughput, non-specific drug discovery assay. The experiment involved binding up a FLAG-peptide sequence-tagged alkaline phosphatase (AP) enzyme with a biotinylated anti-FLAG antibody, in…

  The NPN tenting mechanism I’ve described in a few different posts has relied solely on mechanical technique. I poke the substrate with tweezers, or delaminate it with high electrical forces, or heat it up and let it contract while in…

I have been using ITO electrodes deposited on PTFE sheets for my TEER measurements. A 100 nm thin ITO is deposited on ~300 micron PTFE sheets, and have a sheet resistivity of 60 ohms/sq. This sheet is commercially available from…

I used the following parameters in the Simple Sieving Model (pardon the MATLAB syntax) Am = 1; %cross sectional area in cm^2 Do = 1*10^-7;% diffusion coefficient in the membrane in cm^2/sec P = .15;% membrane porosity; t = linspace(0,420,500);…

Hi all, Currently I am working on a cell culture system to mimic the blood-brain-barrier (BBB). Essentially, growing endothelial cells (bEnd3) on one side of the membrane, and pericyte and/or astrocyte on the other. Below is the layout of the…

I spent some more time in understanding the numbers I am getting for our device simulations. In my simulations, I am assigning a conductivity value for my cell layer. Technically, from this information and by knowing the thickness and area…

Paper can be found here I am working on developing an analytical model to understand how to accurately post-process the resistance values I obtain from my TEER measurements. This paper addresses a similar issue and comes up with a correction factor…

From my last two posts (Anodic Bonding, Ozone Bonding), it is clear that we have some sort of bonding going on between the freestanding membrane and the posts intended to support the membrane. However, we do not understand the nature…