Hi all, Currently I am working on a cell culture system to mimic the blood-brain-barrier (BBB). Essentially, growing endothelial cells (bEnd3) on one side of the membrane, and pericyte and/or astrocyte on the other. Below is the layout of the…

I spent some more time in understanding the numbers I am getting for our device simulations. In my simulations, I am assigning a conductivity value for my cell layer. Technically, from this information and by knowing the thickness and area…

Paper can be found here I am working on developing an analytical model to understand how to accurately post-process the resistance values I obtain from my TEER measurements. This paper addresses a similar issue and comes up with a correction factor…

From my last two posts (Anodic Bonding, Ozone Bonding), it is clear that we have some sort of bonding going on between the freestanding membrane and the posts intended to support the membrane. However, we do not understand the nature…

Hemodialysis chips are not surviving the priming process and the 4 hour dialysis treatment. We want to verify that the membranes are being weakened and also to determine if coating with oxide will ameliorate the problem. Using single slot chips…

We have wondered if we can skip anodic bonding and bond the chips using our UV ozone process. There are pros and cons to this approach, mainly that the setup and procedure is easy, but the contact and alignment is difficult.…

This is an addendum to the results I posted in my previous blog. I am trying to simulate cell growth on a transwell device and perform TEER measurements using a small chopstick electrode pair configuration. I modeled transwell membrane with…

In my previous posts so far, I performed simulations to understand the behavior of TEER variation upon changes in electrode and membrane configuration. None of those models addresses the effects of cell growth on the TEER values. In real experiments,…

In an effort to better understand and check Karl’s model of sieving, I’ve built a significantly simpler version. The goal is to investigate the potential benefits of thinner membranes in dead-end filtration and separations. In my model 1 ml of…

Previous posts: Anodic Bonding Theory, Anodic Bonding Setup   I had originally hoped there would be enough residual impurity in the oxide layer to create a direct bond with Vincent’s chips. This was a foolish hope. Pyrex is at least…