Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and…
Category: memBrains
hiPSC Differentiation into Neural Crest Stem Cells
Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and…
Human Umbilical Vein Endothelial Cell Shear on Manufactured Hybrid (Dual-Scale) Membranes
Introduction In our labs previously work, difficulties in growing endothelial cells (ECs) under shear flow lead to the exploration of novel membrane technologies that permit firm EC attachment, without compromising membrane permeability or leukocyte transmigration studies. We ultimately landed on…
Microfluidic Permeability Assay for In Situ Monitoring of Endothelial Barrier Properties
Introduction As part of my thesis, I have been working on developing an assay for measuring vascular barrier properties within our microfluidic devices. Traditionally, small molecule permeability measurements are performed on cells plated in a Transwell cup. The basic protocol…
