CRE_Bispecific_Antibody_CRS_and_ICANS_Neurotoxicity_Guidance_Feb2024 51899285fnl_Immunotoxic Potential of PharmaceuticalsWe are considering Bispecific T cell engagers (BiTEs) [1] as an alternative to a CAR T driven cytokine release as an immunotherapy focused DDT. BiTEs are monoclonal antibodies engineered to have one arm that recognizes a…
Category: TRACE
IMR90 EECM-BMEC-Like Cells Lack P-gp Efflux Activity
Introduction During the January TraCe-bMPS progress meeting, Jon Flax presented some interesting findings in the iPSC-derived extended endothelial culture method brain microvascular endothelial cell-like cells (EECM-BMECs, hereafter referred to as BMECs) [1]. While these BMECs tended to have high expression…
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Establishing Flow Bumper Supported Basal Flow in Incubator & Best Practices
Introduction The modular µSiM device allows for active flow and continuous monitoring, but the acrylic-needle interface may experience incomplete sealing over longitudinal studies, so the need for an adaptable and intuitive approach for leak-free bottom-channel flow arose. Jordan Jones, Ahmet…
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Modeling Basal Flow in Comsol
Introduction Our goal was to develop a COMSOL model for the bottom channel of the µSiM that can be accessed and easily used by anyone with a COMSOL license. Previously developed simulations for the µSiM rely on the CAD import…
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μSiM Vasc-SkM update 2024.03.13
The μSiM Vasc-SkM Microphysiological development can be separated into 4 parts: Media tuning for integrated culture Force sensing element setup Assembly of the μSiM Vasc-SkM and setting up the perfusion Transendothelial Electrical Resistance (TEER) measurement of the Vast component Work…
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On-line Resolution Calculator Applied to Etaluma Objectives
The following site may be useful to draw to people’s attention when specifying objectives on the Etaluma: https://www.microscope.healthcare.nikon.com/microtools/resolution-calculator/#Focus I have configured a custom resolution calculation using the sensor data supplied by Etaluma, here exemplified at 10x/0.3NA: To take account of…
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