We know: W683 has no visible pores, W685 has usual porosity, and the recent Wafer 747 is ridden with pinholes. I wanted to test this new wafer for discoloration in comparison to the other two and got the expected result.…
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Sepcon-transwell Discoloration IV (2D vs 3D)
Again a repeat, with comparison to the 2D format. To me, it looks like its again a repeat of what we saw in the previous trials. The sample with both intact slits has slightly higher discoloration than the others and…
Sepcon-transwell Discoloration III
Again a repeat of experiment and the observations. As seen above, the sample with both intact slits discolors the fastest (in Sepcon w/memb side down, DMEM w/o FBS, Oven @ 37C). Result is same as the previous two trials. One…
Sepcon-transwell discoloration II
I repeated a previous experiment but this time I used samples (no RTP) from W685 in oven @ 37C in DMEM(-FBS). Four type of samples (blank chip with no slits, chip with both intact membranes, chip with one intact membrane…
Porosity vs Discoloration I
We have known, W683: no visible pores; W684: very few pores; W685: usual porosity. After Vlassak’s view on discoloration, I took samples from above wafers to check if any difference in discoloration can be seen as they have different porosities.…
Sepcon-transwell discoloration study I
In an earlier post we saw that intact membrane sample discolored slower than the broken one. To establish this, a study using four types of samples from #702 (blank chip with no slits, chip with both intact membranes, chip with…
Bead Assay in Sepcon
I carried out the bead assay to monitor membrane integrity in Sepcon format. The sample (no RTP) was taken from #703 and put in DMEM(-FBS) @ RT with membrane side facing up. 15um polystyrene beads were allowed to settle on…
Flipped sample discoloration in Sepcon
We have seen before that in petri dishes, flipped samples (well side up) discolor faster as compared to the ‘membrane side up’ samples. This could be due to local environment trapped below the chip. Hence, I used Sepcon format so…
Discoloration in flipped sample – II
I repeated the experiment with samples from #703. Two samples were in a dish with one flipped over. Again, the ‘well side up’ sample (flipped) discolored earlier as seen in the 24 hour time point in the figure below.
Discoloration in a flipped sample
I am not sure what prompted me to carry out this experiment. I took few samples from W703 (No RTP) and put them in petri dishes with DMEM (-FBS) at room temperature. I had two samples in a dish, with…
