HUVEC adhesion was tested on different substrates. This time solid chips w/o membrane slits were used and expected results were obtained. I think from all the adhesion studies done, it could be concluded that initial adhesion on pncSi is as…
HUVEC adhesion was tested on different substrates. This time solid chips w/o membrane slits were used and expected results were obtained. I think from all the adhesion studies done, it could be concluded that initial adhesion on pncSi is as…
HUVEC adhesion was measured on glass, tissue culture plastic, and pnc-Si, with poly-lysine coated plastic and teflon coverslips as positive and negative controls. Adhesion on pnc-Si was low, this could be due to cells being lost through broken slits or…
Fibroblast adhesion experiment was done using poly-lysine coated plastic and teflon coverslips as positive and negative controls repectively. Poly-lysine is a polypeptide that gives the cells a sticky surface to adhere to. Teflon coverslips are used as negative control because…
Four more adhesion experiments were carried out and the results can be seen in the following charts. Based on the average of the four trials it can be elucidated that the initial adhesion (after ~7 hours) is pretty much the…
Two more adhesion tests were run recently, one on Tuesday and one on Wednesday. For both tests cells were imaged after five hours. Fibroblast trial 3 was the first test where the optimal cell density (assuming 100% of cells are…
Thus far three HUVEC and two L13T3 growth tests have been conducted on substrates including glass,pnc-Si, tissue culture plastic, and untreated plastic. Only one test with untreated plastic has been run for each cell type. Cells on Si samples are…
Adhesions tests are being performed to see if cellular attachment varies between our material, glass, and plastic. These experiments are motivated by lower initial cell densities observed on pnc-Si in growth tests; we are investigating if the lower initial densities…
A second fibroblast (L1 3T3, P21) growth test was conducted on RTP treated samples from W510, tissue culture plastic (polystyrene), and glass cover slips. Fibroblasts were cultured in DMEM with media changed every 48 hours. Cells were imaged using the…
(Transferred from old blog) From the above discoloration array it can be inferred that the RTP treated samples in the incubator are discoloring in much slower rate than the ones on the benchtop. Thus, incubator conditions prevent the chips from…
(Transferred from old blog) The above TEM images show that RTP (800C, 5 min) treated samples (from W334) have their pores intact in salt over a period of time! 1M and 2M NaCl solutions were used and TEM samples were…