We have seen that discoloration of chips is slower in Sepcons and this has been speculated to be because of the separation between the ‘well side’ and the ‘membrane side’ of the chip existing in the Sepcon format. To further…
Author: Anant Agrawal
HUVEC spreading on pncSi and glass
Following time lapse movies show the spreading of HUVECs on pncSi and glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~4 hrs. HUVEC spreading on pncSi HUVEC spreading on glass
Rhodamine diffusion profiles for non-rtp/rtp pncSi
Following images show the diffusion profile of rhodamine across pncSi as observed in the timelapse movies. Looking at the profiles no significant difference is observed between the non RTPed and RTPed samples. Non RTP sample: RTP sample:
Fibroblasts spreading on glass
I carried out another trial of spreading experiments and following time lapse movies show the spreading of fibroblasts on glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~7 hrs. Fibroblast spreading on…
Cell Spreading on pncSi and glass
Spreading of cells (huvec and fibroblasts) on pncSi and glass was observed at ~37 C in L15 media (+ 10% FBS) for ~5 hours. Its observed that the cell spreading is pretty similar on both the substrates and the time…
Rhodamine 6G diffusion through RTP treated and non-treated pncSi (w/o pinholes)
Diffusion of rhodamine was observed through pncSi samples from W620. This was done to compare the porosity of the membranes after RTP treatment. From the following movies (real time = 10 mins) it can be seen that there is no…
Fibroblast spreading on the membrane
Time lapse movie of fibroblast spreading on the nanomembrane was captured over a period of ~ 8 hours. I was having some problem uploading the video here, thus have done so in the NRG knowledge page under the cell culture…
HUVEC adhesion update
Couple of HUVEC adhesion trials were carried out and the results were found satisfactory as seen in the charts below.
HUVEC growth kinetics with serum free media as control
HUVEC growth was monitored for 4 days on different substrates (glass, plastic and pncSi), this time in media (EGM) with and without serum. Growth in media w/o serum was observed to be much slower than the growth in media with…
HUVEC Adhesion with controls (repeat)
HUVEC adhesion was tested on different substrates. This time solid chips w/o membrane slits were used and expected results were obtained. I think from all the adhesion studies done, it could be concluded that initial adhesion on pncSi is as…
