The basics of this device are… Blood flows through a filter made of zeolite containing nanofibers. While the blood flows transversely through the mesh, creatinine can diffuse through the mesh where zeolites stand by to grab them via size selecting…
Author: Dean Johnson
Dr. Johnson is a Research Assistant Professor in Nephrology who was awarded an R01 in 2020. He earned his Ph.D. in Microsystems Engineering from RIT and is currently working on hemodialysis devices based on the ultrathin nanoporous membranes developed earlier by this group.
Dialysis Chip Recovery After Membrane Damage
Josh Miller came up with the idea of filling individual damaged membrane channels with PDMS so the rest of the chip could be salvaged. We worked on it this week, mostly Josh, and we developed a process using strips of…
Posted in NRG
Small Animal Model Dialysis: Nonporous and Microporous Membranes
On Wednesday Feb. 12 and Wednesday Feb. 19, limited exposure dialysis was performed on rats. In the first experiment, blood was pumped with the peristaltic blood pump through a two-chip dialysis device in an acrylic fixture. The membranes were non-porous…
Posted in NRG
Preliminary Small Animal Dialysis Experiments
The overall aim of the small animal dialysis is to evaluate the dialysis membranes and compare their urea clearance to commercial materials. The specific aim of this study was to go through the process of ordering and receiving rats, setting…
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Subscripts and Superscripts
Helpful Hint: H<sub>2</sub>O typed as text will yield H2O visually, and E = mc<sup>2</sup>O typed as text will yield E = mc2. Of course this affects line height, so choose your poison, I guess.
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Interesting Paper: Bioengineered Rat Kidney
Earlier this year an MIT/Harvard group generated and transplanted a rat kidney and published in Nature Medicine Technical Report. “Regeneration and experimental orthotopic transplantation of a bioengineered kidney” Jeremy J Song, Jacques P Guyette, Sarah E Gilpin, Gabriel Gonzalez, Joseph…
Nanoporous Nitride Membranes: 400-µm wide channels, a pictorial survey
Images of the nanoporous nitride wafer. The same wafer that Josh declared as having 30% porosity with an average pore size of 80 nm (or something similar). Josh put images and such on basecamp for those who have access. These…
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Small Animal Model Hemodialysis Set-Up
Sprague-Dawley rats will be purchased from Harlan. These pre-manipulated animals will have been catheterized prior to shipment. We will feed them an adenine rich diet to induce uremia, high levels of urea in the blood. Once uremic, a rat will…
Review: Blood Purification 2013
In vitro Clearance and Hemocompatibility Assessment of Ultrathin Nanoporous Silicon Membranes for Hemodialysis Applications Using Human Whole Blood Morteza Ahmadi Maud Gorbet John T.W. Yeow Department of Systems Design Engineering and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ont. , Canada Summary Two…
Multi channel, dual chip dialysis device
A two multi-channel chip device was fabricated with gaskets and PDMS caps. The Upper fluidic
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