I am trying to grow HUVECs under flow in my device to establish a tight and confluent monolayer for TEER analysis. My device schematic looks like this and the whole setup is as below I tried to use the NPN…
Tag: devices
Cell growth in micovasculature mimetic device
Posted in NRG Tagged with: cell culture, closed flow system, devices, microporous nitride, tissue barrier models
Review: Blood Purification 2013
In vitro Clearance and Hemocompatibility Assessment of Ultrathin Nanoporous Silicon Membranes for Hemodialysis Applications Using Human Whole Blood Morteza Ahmadi Maud Gorbet John T.W. Yeow Department of Systems Design Engineering and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ont. , Canada Summary Two…
Long Term Urea: Evaporation measurement and Pressure Calculations.
The initial measurements with multiple tests tubes containing either Serum or PBS failed to demonstrate evaporation and in fact the weights of the collection tubes increased after sitting in the refrigerator overnight. Perhaps the tape collected water? At any rate…
Counter Flow Dialysis Device Design
Upper and lower gaskets used to make a counter flow device. The danger is that the enclosing pdms can easily flex into the membranes on the flat side of the chip. A hard plastic cover can be used to prevent…
Multiple Chips packaging Concept
While my focus will be on packaging a single multi-channel chip device, I have captured here an initial concept for packaging multiple channels. The technique required is the application of verticle PDMS structures to direct blood and dialysate to and…
Rapid Prototype of Single Channel Dialysis Device
In order to rapidly test the entire setup for dialysis evaluation, including piezoelectric micro motor and recirculating fluid path, a means of quickly evaluating PDMS designs is needed. A mold was created with a multi-print technique in which printer toner…
