This is a cursory review of the nonotechnology articles appearing along with our paper in the Journal “Advances in Chronic Kidney Disease” Most of these are reviews and most deal with nanoparticles for either imaging or therapy. One does discuss…
Author: Dean Johnson
Dr. Johnson is a Research Assistant Professor in Nephrology who was awarded an R01 in 2020. He earned his Ph.D. in Microsystems Engineering from RIT and is currently working on hemodialysis devices based on the ultrathin nanoporous membranes developed earlier by this group.
Hemodialysis in the rat animal model
I found some good descriptions of hemodialysis experiments using rats. They all use a method possibly first described by (Rockel et al., 1978). (Galons, Trouard, Gmitro, & Lien, 1996) at U of Arizona used nephrectomized male Spraque-Dawley rats (Holtzmann Co.,…
Hemo biometrics for various animals
In determining an appropriate animal model for hemodialysis efficacy, several metrics need to be compared to the human vasculature. Blood volume, blood pressure, and heart rate could effect the suitability of these animals to model clinical hemodialysis. Blood volume can…
Urea/f-BSA/cytochrome c concentrations in retentate after dialysis: 12 hours
Continuing on the path of hemodialysis, we are increasing the complexity of the fluid being dialyzed. In this long-term experiment I will pump 100% serum with added urea (1mM) cytochrome c (1 mg/mL) and fluorescent BSA (1 mg/mL). So I…
Long Term Urea: Evaporation measurement and Pressure Calculations.
The initial measurements with multiple tests tubes containing either Serum or PBS failed to demonstrate evaporation and in fact the weights of the collection tubes increased after sitting in the refrigerator overnight. Perhaps the tape collected water? At any rate…
Schematic of Long Term Clearance Test Setup.
This is the setup used for the long term clearance testing. The fraction collector can be set to advance to the next collection tube at set intervals from 1 min to 99 min. I am currently conducting an evaporation test…
Long Term Urea Clearance in 100% Serum
I ran a 30 hour urea clearance with 100% serum through the single slot dialysis device. The now standard protocol was run. The single slot chip was annealed in the RTP for robustness then clamped into the acrylic/PDMS flow fixture.…
Summary of Fissell's review paper in 2013 International Kidney
Quick review of “Achieving more frequent and longer dialysis for the majority: wearable dialysis and implantable artificial kidney devices” by Fissell, Roy, and Davenport see Fissell(Roy)_2013_International_Kidney.pdf in the hemodialysis section of the library. First a little background: Two type of…
Posted in NRG
Paper Review… Human Nephron Filter: Nissenson(Watts) UCLA
In researching for the paper for Advances in Chronic Kidney Disease (ACKD) I’ve run across some interesting papers. Here is one describing a proposed Human Nephron Filter… Human nephron filter proposed by Nissenson1 (1) 1Division of Nephrology, Department of Medicine,…
Implantable Kidney
DailyHemo Report of Dr. Fissell’s Implantable Kidney Development Sometimes the link above “Implantable Kidney” doesn’t seem to work. http://www.dailyhemo.org/implantable-kidney
Posted in NRG
