Author: Jim
Professor McGrath holds a BS degree in Mechanical Engineering from Arizona State and a MS degree in Mechanical Engineering from MIT. He earned a PhD in Biological Engineering from Harvard/MIT's Division of Health Sciences and Technology. He then trained as a Distinguished Post-doctoral Fellow in the Department of Biomedical Engineering at the Johns Hopkins University. Professor McGrath has been on the Biomedical Engineering faculty at the University of Rochester since 2001 where he also served as the director of the graduate program in BME for more than a decade and currently serves as Associate Director of the URNano microfab and metrology core. Professor McGrath also has faculty affiliations with many other programs at UR including the Material Research Program, the Environmental Health and Sciences Center, the Biochemistry and Biophysics program, and the Musculoskeletal Research Center. McGrath's graduate, post-doctoral, and early faculty research was focused on quantitative experiments and mathematical modeling of cell migration covering molecular, cellular, and multi-cellular phenomena. This was true until 2007 when he, along with Professor Philippe Fauchet (now Dean at Vanderbilt) and PhD students Tom Gaborski (RIT) and Chris Streimer (Adarza), discovered a means to self-assembled nanopores in 15 nm thick free-standing silicon and demonstrated the remarkable transport properties of the new material in a Nature paper. This seminal discovery led to the creation of the multidisciplinary Nanomembrane Research Group (NRG) and the founding of SiMPore Inc. in the same year. The NRG and SiMPore have been dedicated to the advancement of ultrathin membrane technologies and exploring all of their potential applications ever since. This blog also dates back to 2007 and has had contributions from more than 100 students, faculty, scientists, engineers, and entrepreneurs. It contains over 2,500 pages and posts logging progress large and small over all these years. Yet somehow it feels like we are just getting started.

Super useful conversions between volumes and lengths

1 m^3 = 1000 L 1 cm^3 = mL 1 mm^3 = uL 1 um^3 = fL 100 µm x 100 µm x 100 µm = 1 nL 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1

Posted in Knowledge (Public)

Evidence of functional sodium/hydrogen exchangers in single EVs captured on NPN

Thanks to our collaborators at the University of Chicago we have now used NPN to demonstrate sodium/hydrogen exchange functions at the level of single EVs. This is first-of-a-kind evidence of protein function in individual EVs. It will be officially published

Posted in NRG

CRePE-ssNP UR Update Nov. 2021

To keep the NRG up-to-date on the goings-on in our collaboration with the University of Ottawa, we will plan to share our progress slide decks from the CRePE-ssNP group meetings that happen every two months. Periodically, we’ll also update the

Posted in NRG

R61: 12 Month Progress to NIH Slide Decks

R61; 12 month Progress (Rochester) Small Trans-Agency Blood-Brain Interface Program Investigator Meeting – Agenda_FINAL

Posted in memBrains

Thinkin’ Secretion in the µSiM

One of the many contributions Alec S. made to our group is to help us appreciate the importance of the apicobasal (A/B) polarity of endothelial cells. The effects of A/B polarity were demonstrated in his paper: Integrative Biology (2020), 12(11),275–289

Posted in memBrains, NRG, μSiM

Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 4: Sampling)**

Introduction In addition to in situ measurements of small molecule permeability (Parts 1, 2 and 3), we wish to enable an assessment of permeability by sampling from the abluminal chamber of the µSiM in a manner similar to what is

Posted in memBrains, NRG

Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 3: Application to Cell Barriers)*

This is the third installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position

Posted in memBrains, NRG, μSiM

R61: Slide Decks for 6 Month Progress Report

PDF with all slides: R61 HL154249-01(6 Month Progress)

Posted in memBrains

Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 2: Experimental Validation)

This is the second installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position

Posted in memBrains, NRG, μSiM Tagged with:

Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 1: Approach)

NOTE: Some of the ideas in this post are borrowed and rephrased from prior posts by Molly and Alec and our paper Khire et al. 2020. The purpose of this thread is to explain things anew with an eye on

Posted in memBrains, NRG Tagged with:
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