1 m^3 = 1000 L 1 cm^3 = mL 1 mm^3 = uL 1 um^3 = fL 100 µm x 100 µm x 100 µm = 1 nL 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1…
1 m^3 = 1000 L 1 cm^3 = mL 1 mm^3 = uL 1 um^3 = fL 100 µm x 100 µm x 100 µm = 1 nL 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1…
Thanks to our collaborators at the University of Chicago we have now used NPN to demonstrate sodium/hydrogen exchange functions at the level of single EVs. This is first-of-a-kind evidence of protein function in individual EVs. It will be officially published…
To keep the NRG up-to-date on the goings-on in our collaboration with the University of Ottawa, we will plan to share our progress slide decks from the CRePE-ssNP group meetings that happen every two months. Periodically, we’ll also update the…
R61; 12 month Progress (Rochester) Small Trans-Agency Blood-Brain Interface Program Investigator Meeting – Agenda_FINAL
One of the many contributions Alec S. made to our group is to help us appreciate the importance of the apicobasal (A/B) polarity of endothelial cells. The effects of A/B polarity were demonstrated in his paper: Integrative Biology (2020), 12(11),275–289…
Introduction In addition to in situ measurements of small molecule permeability (Parts 1, 2 and 3), we wish to enable an assessment of permeability by sampling from the abluminal chamber of the µSiM in a manner similar to what is…
This is the third installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position…
PDF with all slides: R61 HL154249-01(6 Month Progress)
This is the second installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position…
NOTE: Some of the ideas in this post are borrowed and rephrased from prior posts by Molly and Alec and our paper Khire et al. 2020. The purpose of this thread is to explain things anew with an eye on…