I recently tested the burst pressures of samples from W510, 511, and 513. None of the membranes are pinhole free. Silicon thickness varies (15 nm, 10 nm, 20 nm). See Dave’s post for complete fabrication information. W513 has noticeably higher…
Author: Karl Reisig
Fibroblast Growth: Viability and Discoloration
I recently attempted a fibroblast (L1 3T3 cell line) growth test. The cells were seeded at too high of a density, so growth curves can’t be produced. However I checked cell viability and pnc-Si discoloration. As expected RTP treated samples…
Posted in NRG
Pinhole Study: W510
To improve our knowledge of pinhole formation I have started counting pinholes on W510, W512, and W513. I also am playing close attention to areas surrounding the samples that have been completely etched, to see if this correlates to pinhole…
HUVEC Growth on Si: Take 2 and 3
Last week I attempted another HUVEC growth study on Si, coverslips (glass), and culture wells (plastic). The cells were seeded at too low of a density on the membrane so I adjusted my protocol and started a third growth test.…
Incubator versus Benchtop
(Transferred from old blog) From the above discoloration array it can be inferred that the RTP treated samples in the incubator are discoloring in much slower rate than the ones on the benchtop. Thus, incubator conditions prevent the chips from…
Effect of salt on pores (benchtop)
(Transferred from old blog) The above TEM images show that RTP (800C, 5 min) treated samples (from W334) have their pores intact in salt over a period of time! 1M and 2M NaCl solutions were used and TEM samples were…
RTP Treated Membrane Stability in DMEM
(Transferred from old blog) Wafer 303 samples, both non-RTP and RTP (800C for 5 minutes), were tested in cell media (DMEM supplemented with 10% FBS) or deionized water, and placed in the incubator (37C 5% CO2) or oven (approximately 37C).…
pH Variation of DMEM
(Transferred from old blog) Aim: Determine how quickly DMEM pH changes once removed from the incubator Methods and Materials: • non-sterile complete Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS • 6 and 24 well cell culture plates •…
W501: Air Permeability and Burst Pressure
Here are the results for air permeability testing of W501. The sample closest to the origin had one very small (very difficult to see on Brian’s scope unless viewing at 100x) pinhole, the middle sample had 3 very small pinholes.…
Posted in NRG
HUVEC Growth on Si
Over the past five days Anant and I monitored human umbilical vein endothelial cell (HUVEC) proliferation on three substrates: RTP-treated Si samples (both with and without membranes), glass (microscope coverslips), and plastic (6-well tissue culture plates). After the first two…
