Introduction This post will describe the preliminary work I have completed using size exclusion chromatography (SEC) for EV enrichment. We are ultimately interested in interrogating serum EV biomarkers using CAD-LB, however, the presence of protein and lipoprotein contaminants reduces the…
Author: Sam Walker
CAD-LB: Biomarker Detection Validation – ICAM-1 HUVEC Stimulation Model
Introduction This post is another update in my series of experiments aiming to both characterize and validate the CAD-LB platform. We have now shifted our focus to validating CAD-LB biomarker detection which will be done using multiple experimental models. The…
CAD-LB: Colocalization Detection Guide
Introduction In this post I will review work I have completed to further characterize the CAD-LB platform. In this set of experiments combinations of nanoparticle species were used to define colocalization limitations. More specifically, two nanoparticle species each labeled with…
Characterization of the CAD-LB: Dynamic Range & Capture Efficiency
Introduction This post is meant to serve as an update for some of the more recent work completed on the CAD-LB (catch and display for liquid biopsy) platform. The CAD-LB is intended to be used as a digital biomarker assay…
Ibidi (Rev B) Device
Introduction This post is meant to introduce the new NPN-enabled slide-based devices produced by Ibidi. These devices feature an NPN membrane and microfluidic channels positioned perpendicular to one another formed by PSA and COP layers (Figure 1). Channels above and…
Posted in NRG
Immune Checkpoint Therapy – EV Analysis
Introduction & Motivation A subset of cancer cells has mutations that generate proteins recognized by the immune system as foreign, triggering the immune-mediated clearance of those cells. To avoid this fate, cancer cells can evolve mechanisms to suppress this antitumor…
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ALine for EV Capture
ALine Capture Device In this post I will go over some of my recent work that is a continuation of Kilean’s research concerning extracellular vesicle (EV) capture and characterization. In the past, this was done using a tangential flow filtration…
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Plasma Problems
Introduction This post will detail the work completed in the last chapter of my Thesis. The overarching goal of this chapter was to perform dialysis on whole blood utilizing a serial-separation system. This system would first: generate plasma from whole…
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Parylene Treatment Effect on Membrane Burst Pressure and Permeance
Introduction & Background Parylene is a polymer stemming from the chemical-vapor-deposited poly(p-xylylene) group. This polymer treatment is applied as a gas which allows complete and uniform coatings of substrates with even nanoscale features. Parylene treatment is widely used as it…
Double Slot: Single-Pass Dialysis Devices Update
This post is meant to conclude the work that I have completed this summer with the double slot single pass devices; recently I have conducted additional experiments and collected ultrafiltration data. In my previous post, “Single Slot Inverted-Chip and Double…
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