I am trying to grow HUVECs under flow in my device to establish a tight and confluent monolayer for TEER analysis. My device schematic looks like this and the whole setup is as below I tried to use the NPN…
Tag: closed flow system
Cell growth in micovasculature mimetic device
Posted in NRG Tagged with: cell culture, closed flow system, devices, microporous nitride, tissue barrier models
Microfluidics in Hemodialysis Project
The purpose of the fluidics in our hemodialysis system is to move the sample to and the retentate away from the membranes. The dialysate (permeate, filtrate) will be handled passively in the initial designs and therefore do not require fluidic…
The Whitesides Chamber implemented in the McGrath Lab has now successfully generated a steady and linear gradient
Hi all, After nearly a month of troubleshooting the fabrication of Whitesides Chamber we finally have the capability to generate a steady and linear gradient of molecules. In the presented case, we have generated a gradient of rhodamine 6G (R6G),…
Posted in NRG Tagged with: characterization, closed flow system, diffusion, diffusion cell design, flow cell, henry, micropump, rhodamine diffusion
Materials in contact with media in micropump
The pump’s data sheet (located here) describes the pumps materials as: polyphenylene sulphone (PPSU) polyimide (PI) nitrile butadiene rubber (NBR) It also says that the acceptable particle tolerance is < 50 μm. This is the info we will need to…
15um Bead Flow through Closed Flow Cell
Based on the results obtained yesterday with the closed flow cell we reran another trial today. The closed flow system we tested today was bound using UV Ozone treatment, surprisingly this system created a relatively strong bond despite our resent…
No success with UV Ozone Bonding at 100C with High Humidity
Following the advice of Dave and Chris I ran a UV Ozone bonding trial yesterday with the stage temperature set to 100C (instead of the usual 25C). The idea behind the elevated stage temperature is to drive off any water…
