The following are TECAN spectrum of the filtrates (and a spectrum of unconjugated peptide) for a series of sequential separations performed on the Barcikowski peptide (CE12W) using 10, 20, and 30nm cutoff Sepcons performed last weekend. Note that all of…
Category: NRG
"Phantom" Curves on the TECAN Mess with Reproducibility and Accuracy
At some point, when things settle down, I’ll create a page dedicated to the TECAN machine and document all of the troubleshooting and tricks I’ve learned so far. Before that happens I have another mystery to solve. Take a look…
Micropump Data Update
After a few more revisions of the micro pump, I performed a significantly more thorough characterization. Flow rate was determined as before using a horizontal capillary of known volume and a stop watch. A extremely small thermocouple…
A quick graph showing that a higher active area/inactive area ratio sees less pore blockage
These volume measures were conducted at 5.6 +/-0.2 PSI using the variable area sepcons with either a half slot, one slot, three slots, or the normal five slots. Note that the pressure was normalized via a pressure regulator, and…
Posted in NRG Tagged with: BSA, pressure, pressure setup, separation, streamline convergence?, TECAN, variable area
PEG Microarray Developments
Updates: We can now put the glass on top of the mask, which allows the use of nonstick agents in the creation of PEG microarrays. The ability to cross-link PEG-Diacrylate (PEG-DA) with a photoinitator and UV exposure was previously tested and…
Posted in NRG
CytoVu Static TEER Device with Yuchen Wang
Yuchen and I have been tasked with integrating TEER electrodes into the Cytovu format. The general idea was to fabricate the TEER electrodes similarly to what I have done already, then add them to the current Cytovu device, using UV…
Posted in NRG
40nm Gold Passes Through 40 nm pnc-Si Sepcon Chips; 100nm Fluorospheres Do Not.
On Friday Josh and I assembled Sepcon chips with calculated porosity of ~6.5 %, and avg. pore diameter of ~40 nm into Sepcons. I was able to pass 40nm gold through the pores (we don’t have any gold larger than that). Note…
Posted in NRG
Lab Specific Training
Please fill out this Lab Specific training form, sign and return to me. If the item is not applicable to your work, sign N/A. Jim LabSpecificTraining(2013).pdf
Posted in NRG
Temp-controlled 'nanopores' may allow detailed blood analysis
http://phys.org/news/2013-03-temp-controlled-nanopores-blood-analysis.html
Posted in NRG
TECAN absorbance for 96-well plates is topping out even with pure buffer
EDIT: This problem was solved thanks to Tejas. It turns out that Falcon BD transparent 96 well plates absorb in the UV range, and are best used for bradford assays or anything that measures absorbance higher than the UV range.…
