video (this version is saved to the server. It’s smaller and blurrier) http://www.youtube.com/watch?v=2JZ8uTWhejs&feature=youtu.be (this version is private – it can only be accessed by people with the exact link – it’s high quality) I modified the bottom basket of a Sepcon…
Category: NRG
Nanopores May Eliminate Current Evaluation Processes Used for Detecting Epigenetics-Related Diseases
http://www.azonano.com/news.aspx?newsID=26865
Posted in NRG
Permeability Graphs for BSA Separations with Variable-Active-Area Sepcons Show Flow is Independent of Fractional Active Area
What I had in my two previous posts described as permeability graphs were in fact total volume passed/ total time passed graphs: What I thought I saw was that cake layer formation was dependent on the filter geometry. There is…
How water sticks to surfaces
Tejas found this… A cool article and video on water ‘sticking’ the surface. Notice the difference in advancing and receding angles. http://web.mit.edu/newsoffice/2013/droplet-surface-adhesion-0219.html
Posted in NRG
A New Round of Separations Still Shows an Increase in Filtrate Flux Above Normalization for Larger Active Area Sepcons
Using methods pretty much identical to those outlined in Tuesday’s post, I was able to get very similar data for a different BSA concentration. Whereas Tuesday’s curves were all done with 1mg/mL BSA, this was done with 0.5mg/mL BSA. The first…
Posted in NRG
Barcikowski Particles Revisited
The following are TECAN spectrum of the filtrates (and a spectrum of unconjugated peptide) for a series of sequential separations performed on the Barcikowski peptide (CE12W) using 10, 20, and 30nm cutoff Sepcons performed last weekend. Note that all of…
Posted in NRG
"Phantom" Curves on the TECAN Mess with Reproducibility and Accuracy
At some point, when things settle down, I’ll create a page dedicated to the TECAN machine and document all of the troubleshooting and tricks I’ve learned so far. Before that happens I have another mystery to solve. Take a look…
Micropump Data Update
After a few more revisions of the micro pump, I performed a significantly more thorough characterization. Flow rate was determined as before using a horizontal capillary of known volume and a stop watch. A extremely small thermocouple…
A quick graph showing that a higher active area/inactive area ratio sees less pore blockage
These volume measures were conducted at 5.6 +/-0.2 PSI using the variable area sepcons with either a half slot, one slot, three slots, or the normal five slots. Note that the pressure was normalized via a pressure regulator, and…
Posted in NRG Tagged with: BSA, pressure, pressure setup, separation, streamline convergence?, TECAN, variable area
