To complement the recent study which investigated pore changes during discoloration with TEM, I did AFM on the same samples. These were w673 samples which were incubated in DMEM (-FBS) in the 37C oven. We didn’t use FBS because I…
Category: NRG
RTP + Silanization (3D format)
As seen before, in 2D format ‘RTP+silanization’ helps. Similarly in 3D format, with membrane side down, the same treatment helps prevent discoloration till 5 days. (samples from W413) Above figure shows how combined effect of RTP and silanization is better…
AFM – wafer characterization
Chris gave me a few wafers to look at with AFM – an oxide sputtered in the recently cleaned chamber and VA Semiconductor wafers cleaned with “Normal Power” or “Full Power” Megasonics. I employed a couple of new “tricks” to…
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Pore processing of DMEM incubated samples
In continuation of the previous post, I ran the pore processing software for two samples (t=0 and t=150 min). The following histograms show the pore parameters and the difference in the pore size and numbers between the two samples. I…
TEM images of pnc-Si incubated in DMEM(-FBS)
There has been uncertainty with process of dissolution of pnc-Si in cell growth media. Whether the media enters the pores and ‘eats away’ the material leading to increase in pore sizes and eventually complete degradation was the question. Thinning of…
New Wafers
A few of the newest SC wafers came back with intact samples without any pinholes. First here is a list of the experiments that we want to do in our lab with intact/pinhole free membranes 1. Protein separations – Requires…
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AJA RTP temperature series
In the last few weeks, the production team has focused on making strong, stable, porous material with the AJA sputter system. In the process, we’ve RTPed a wafers over a wide range of temperatures under different deposition conditions. We found…
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Discoloration in cell culture conditions
Lately, Anant and I have been doing our discoloration experiments under purposely harsh conditions so we don’t have to wait for multiple days for experiments to finish. We have lots of evidence that the 3D format slows down discoloration (post1,…
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RTP + Silanization
After recent studies with surface treatments, we wanted to take a step further by trying amino silanization on RTP’ed samples. Wafer 413 samples were RTP’d with no intact slits. Both 2D and 3D formats were tested in DMEM (-FBS) in…
Rhodamine transport in pnc-Si and PET transwells
This is some preliminary data that I put into my F32 postdoc fellowship application. It is a comparison of rhodamine transport through pnc-Si and PET transwells. These studies were done with PBS as the buffer. Sample volume = .1mL, donor…
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