There has been uncertainty with process of dissolution of pnc-Si in cell growth media. Whether the media enters the pores and ‘eats away’ the material leading to increase in pore sizes and eventually complete degradation was the question. Thinning of…
Category: NRG
New Wafers
A few of the newest SC wafers came back with intact samples without any pinholes. First here is a list of the experiments that we want to do in our lab with intact/pinhole free membranes 1. Protein separations – Requires…
Posted in NRG
AJA RTP temperature series
In the last few weeks, the production team has focused on making strong, stable, porous material with the AJA sputter system. In the process, we’ve RTPed a wafers over a wide range of temperatures under different deposition conditions. We found…
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Discoloration in cell culture conditions
Lately, Anant and I have been doing our discoloration experiments under purposely harsh conditions so we don’t have to wait for multiple days for experiments to finish. We have lots of evidence that the 3D format slows down discoloration (post1,…
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RTP + Silanization
After recent studies with surface treatments, we wanted to take a step further by trying amino silanization on RTP’ed samples. Wafer 413 samples were RTP’d with no intact slits. Both 2D and 3D formats were tested in DMEM (-FBS) in…
Rhodamine transport in pnc-Si and PET transwells
This is some preliminary data that I put into my F32 postdoc fellowship application. It is a comparison of rhodamine transport through pnc-Si and PET transwells. These studies were done with PBS as the buffer. Sample volume = .1mL, donor…
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Liquid Silanization again
A bunch of samples from W683 (no RTP) were liquid silanized and used in 3D format to track discoloration in comparison to control sample, in DMEM(-FBS) @ 37C in Oven. I used all the cracked chips which broke while clamping…
Silanization and BSA coating
Surface treatments like silanization were seen to slow down discoloration in the past. I carried out liquid amino-silanization (protocol in place) of samples from W683 (no RTP) and used them in both 2D and 3D format to monitor discoloration in…
Discoloration – loose ends & ground up wafer
There were some ideas being thrown around a couple weeks ago about discoloration results. I tested a couple of them out last week. These experiments were with w615 pieces, in the 37C oven in serum-free DMEM. I tested if vacuum…
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High Resolution TEM at UR – fairly easy
I did some imaging on the URinc TEM today and took the nice image below from wafer SC021. I wanted to see the correlation between highly diffracting (dark) crystals in our standard lower resolution TEM and the lattice fringes observed…
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