In an earlier post we saw that intact membrane sample discolored slower than the broken one. To establish this, a study using four types of samples from #702 (blank chip with no slits, chip with both intact membranes, chip with…
Category: NRG
Bead Assay in Sepcon
I carried out the bead assay to monitor membrane integrity in Sepcon format. The sample (no RTP) was taken from #703 and put in DMEM(-FBS) @ RT with membrane side facing up. 15um polystyrene beads were allowed to settle on…
Cell viability – XTT
XTT is a colorimetric assay for measuring cell viability. Supposedly, it gets taken up by cells and is reduced by mitochondrial activity intracellularly into water-soluble orange formazan crystals. The readout is the absorbance of these orange crystals. I used XTT…
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Discoloration CVC vs. AJA Tool
Based on Barrett’s array set up for discoloration, I set up an array experiment to check the discoloration effects between the new and old system ( 2e-7,AJA vs the older 7e-7 CVC ). The wafers from each had a bit…
High res TEMs from UIUC
Here are some high resolution TEM images from a SiMPore-UIUC collaborator. Chris says: The high resolution stuff looks pretty cool, especially the zoom-in on the 8nm pore. I the most obvious crystal planes are <111> with 0.314 nm spacing. If…
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Flipped sample discoloration in Sepcon
We have seen before that in petri dishes, flipped samples (well side up) discolor faster as compared to the ‘membrane side up’ samples. This could be due to local environment trapped below the chip. Hence, I used Sepcon format so…
Discoloration in flipped sample – II
I repeated the experiment with samples from #703. Two samples were in a dish with one flipped over. Again, the ‘well side up’ sample (flipped) discolored earlier as seen in the 24 hour time point in the figure below.
Diffusion Gel Reruns
The movable cutoff gels were more or less successful, but were too dirty for publication (see knowledge page). I’ve been trying to rerun them so that they look nicer, but the darker stain that I used has brought out a…
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Water Permeability Figure
Previous Post We discussed in meeting having Maryna also calculating the permeabilities for this figure. Here is a figure with the experimental, theoretical permeabilities from Dave’s, Maryna’s, and my processing, and an average of the three theoretical permeabilities. I then…
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First TEMs of AJA films
After much anticipation, we have confirmed that pores do form with the AJA deposition system! Below are a series of images of membranes of two different thicknesses that were annealed at several temperatures in the RTP. 1000 C RTP; 40/9/40…
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