Again a repeat, with comparison to the 2D format. To me, it looks like its again a repeat of what we saw in the previous trials. The sample with both intact slits has slightly higher discoloration than the others and…
Category: NRG
Sepcon-transwell Discoloration III
Again a repeat of experiment and the observations. As seen above, the sample with both intact slits discolors the fastest (in Sepcon w/memb side down, DMEM w/o FBS, Oven @ 37C). Result is same as the previous two trials. One…
Updated Water Permeability Figure
In the previous post, I used a compound error bar that took into account both image and operator variability. While that seemed to make things a little better, we were still having a problem with w705. I reprocessed a couple…
Posted in NRG
BBEC Pressurize/Diffusion Separations
I wanted to attempt pressurized and diffusion separations of brain extract using the new movable wafers. I tried pressure cell separations of 1:20 BBEC (clarified brain extract) at 2psi for 40 min. The flow rates were better than the standards…
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Sepcon-transwell discoloration II
I repeated a previous experiment but this time I used samples (no RTP) from W685 in oven @ 37C in DMEM(-FBS). Four type of samples (blank chip with no slits, chip with both intact membranes, chip with one intact membrane…
Porosity vs Discoloration I
We have known, W683: no visible pores; W684: very few pores; W685: usual porosity. After Vlassak’s view on discoloration, I took samples from above wafers to check if any difference in discoloration can be seen as they have different porosities.…
HUVEC 'proliferation' in Sepcons
Last week when I reported no membrane breakage after a week of HUVEC culture, I showed a phase micrograph on day 7. This week I tracked the HUVECs a bit more closely with the microscope. This is 10X, HUVEC P5,…
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Pressurized rerun with w670
Previous Post The previous w670 pressurized flow gels showed one trial with a lower cutoff than w673 and one trial with nothing passing through. This experiment was run for 40 min and in each case I saw only 0-3 uL…
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1st HUVEC TEER data
I recently completed some experiments with the Endohm system, which measures transendothelial electrical resistance (TEER). I seeded either 16.25k or 32.5k HUVECs (P5) on commercial polycarbonate (PC) or polyester (PE) transwells and on pnc-Si in the transwell format. I then…
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Discoloration – Sepcon pieces
Since we’ve been noticing slower discoloration in the Sepcon format, I looked at the 2 components of Sepcons – the plastic and the O-ring. Specifically, I cut pieces from w622 and incubated them in FBS-DMEM/F12 either alone, with the Sepcon…
Posted in NRG
