I repeated a previous experiment but this time I used samples (no RTP) from W685 in oven @ 37C in DMEM(-FBS). Four type of samples (blank chip with no slits, chip with both intact membranes, chip with one intact membrane…
Category: NRG
Porosity vs Discoloration I
We have known, W683: no visible pores; W684: very few pores; W685: usual porosity. After Vlassak’s view on discoloration, I took samples from above wafers to check if any difference in discoloration can be seen as they have different porosities.…
HUVEC 'proliferation' in Sepcons
Last week when I reported no membrane breakage after a week of HUVEC culture, I showed a phase micrograph on day 7. This week I tracked the HUVECs a bit more closely with the microscope. This is 10X, HUVEC P5,…
Posted in NRG
Pressurized rerun with w670
Previous Post The previous w670 pressurized flow gels showed one trial with a lower cutoff than w673 and one trial with nothing passing through. This experiment was run for 40 min and in each case I saw only 0-3 uL…
Posted in NRG
1st HUVEC TEER data
I recently completed some experiments with the Endohm system, which measures transendothelial electrical resistance (TEER). I seeded either 16.25k or 32.5k HUVECs (P5) on commercial polycarbonate (PC) or polyester (PE) transwells and on pnc-Si in the transwell format. I then…
Posted in NRG
Discoloration – Sepcon pieces
Since we’ve been noticing slower discoloration in the Sepcon format, I looked at the 2 components of Sepcons – the plastic and the O-ring. Specifically, I cut pieces from w622 and incubated them in FBS-DMEM/F12 either alone, with the Sepcon…
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Sepcon-transwell discoloration study I
In an earlier post we saw that intact membrane sample discolored slower than the broken one. To establish this, a study using four types of samples from #702 (blank chip with no slits, chip with both intact membranes, chip with…
Bead Assay in Sepcon
I carried out the bead assay to monitor membrane integrity in Sepcon format. The sample (no RTP) was taken from #703 and put in DMEM(-FBS) @ RT with membrane side facing up. 15um polystyrene beads were allowed to settle on…
Cell viability – XTT
XTT is a colorimetric assay for measuring cell viability. Supposedly, it gets taken up by cells and is reduced by mitochondrial activity intracellularly into water-soluble orange formazan crystals. The readout is the absorbance of these orange crystals. I used XTT…
Posted in NRG
Discoloration CVC vs. AJA Tool
Based on Barrett’s array set up for discoloration, I set up an array experiment to check the discoloration effects between the new and old system ( 2e-7,AJA vs the older 7e-7 CVC ). The wafers from each had a bit…
