Fluorescent Streptavidin Coated Bead Capture on Biotin KODE Coated Surfaces After determining that KODE molecules can successfully coat our silicon pore membranes, we set out to determine if the coating could be functional and how stable it was. To test…

 Goal: Is to produce a membrane has pockets to capture the extracellular vesicles EV’s. Membrane structure: Figure 1 shows the membrane structure. The membrane will have two pores sides: upper and lower. The pores on top are bigger than the…

Wound healing experiments I have been isolating extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) and using them in wound healing experiments. For isolation, I follow the same protocol as outlined here. For these experiments, I used small EVs, which…

Goal In preparation for experimentation with hCMEC/D3, an immortalized BBB cells line, I characterized barrier formation by taking TEER. I want to find peak barrier formation to determine what day to run assays. Methods hCMEC/D3 were maintained and passaged in…

In the study, which combines the compressed sensing (CS) and the structured illumination microscopy (SIM) methods, an object plane should first be collected by a lens, and then the collected signal should be measured by a photomultiplier tube (PMT), located…

Hi all,   I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius. Best Comittee Meeting

Summary There’s a lot to be desired in counting pores in grayscale images, especially when the contrast differences are low. A human eye is usually much better at picking out slight differences in contrast, and this can mean the difference…

We previously explored cell migration and cell-substrate interactions in the form of altered f-actin polymerization, focal adhesion formation, and fibronectin fibrillogenesis on the discontinuous substrates (porous membranes and non-fouling PEG islands). Here, we were curious about mechano-sensing on these substrates.…

As a quick update on the ECM masking with Parylene membrane, we made changes to the device designs. Instead of relying on the peel-off process, we used a simplified design, with the membrane serving as the roof of the channels.…

Now that I am working on labeling my 5637 EVs, I have finally gotten around to getting a good labeled sample and a good negative control. The samples were prepared as follows: 5637 EVs were thawed from a frozen stock…