In the study, which combines the compressed sensing (CS) and the structured illumination microscopy (SIM) methods, an object plane should first be collected by a lens, and then the collected signal should be measured by a photomultiplier tube (PMT), located…

Hi all,   I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius. Best Comittee Meeting

Summary There’s a lot to be desired in counting pores in grayscale images, especially when the contrast differences are low. A human eye is usually much better at picking out slight differences in contrast, and this can mean the difference…

We previously explored cell migration and cell-substrate interactions in the form of altered f-actin polymerization, focal adhesion formation, and fibronectin fibrillogenesis on the discontinuous substrates (porous membranes and non-fouling PEG islands). Here, we were curious about mechano-sensing on these substrates.…

As a quick update on the ECM masking with Parylene membrane, we made changes to the device designs. Instead of relying on the peel-off process, we used a simplified design, with the membrane serving as the roof of the channels.…

Now that I am working on labeling my 5637 EVs, I have finally gotten around to getting a good labeled sample and a good negative control. The samples were prepared as follows: 5637 EVs were thawed from a frozen stock…

Introduction Sphingosine-1-phosphate: production and endothelial cell signaling Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid produced through the phosphorylation of sphingosine, a component of the plasma membrane, by SphK1/2. Many cells produce and store S1P, the largest source of which is platelets,…

Introduction EC Apicobasal polarity in immunity has and will continue to be a theme of my thesis. I have presented on bits and pieces of this work over the past year or so, but now that this work is under…

Since precision printing FITC KODE molecules into the windows of our 3 slot chips I have begun testing both the uniformity and the functionality of surface coatings of these molecules. In pursuit of coating uniformity I have tested several different…

This post serves as an introduction to the uni-axial motion envisioned to be integrated into the tendon-on-chip platform. As discussed previously we are working in junction with Dr. Awad from the Center for Musculoskeletal Research (CMSR) center at UR to…