Just wanted to drop this data here in case it may be useful for others in the lab. I calculated the volumetric flow rates for one of the peristaltic pumps used in the lab for the flow circuit in cell-culture.…
Category: NRG
HUVEC polarity to TGF-β1 treatment
Introduction: Transforming Growth Factor -β1 (TGF-β1) is a key player in the induction and progression of fibrosis in many tissues. This factor has also been established as a regulator in the development of fibrotic diseases for its involvement of different…
Posted in NRG
Human Umbilical Vein Endothelial Cell Shear on Manufactured Hybrid (Dual-Scale) Membranes
Introduction In our labs previously work, difficulties in growing endothelial cells (ECs) under shear flow lead to the exploration of novel membrane technologies that permit firm EC attachment, without compromising membrane permeability or leukocyte transmigration studies. We ultimately landed on…
SERS nanostructure imaging in NPN and npMgF2, with heat treatments
Over the last few months, I have been trying to reestablish my SERS measurements, and generate a paper based on SERS sensing with our nanomembranes. Previously, I have created magnesium fluoride coated substrates that seem to have better SERS performance…
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Fabricating an integrated device for virus-detection.
So this is the latest iteration of the thumb-pump device we were working on this summer. As you will see in the diagrams, the idea is to ultimately have saliva go through a filtration step, with a side channel leading…
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Sartorius Internship Results – Last Aim of My Ph.D.
Hi all – I finished my 10 months internship with Sartorius on 16th of July and since all my results are going to be the third and last aim of my Ph. D., I will be presenting my results in…
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Size Exclusion of the Vasculature – Polarized TNF-alpha Revision Experiment
Background Previously we characterized HUVEC monolayer barrier via TEER in traditional transwell systems following polarized TNF-alpha exposure (Figure 1). One major shortcoming of this experimental set up for our study is it does not accurately reflect the permeability of HUVECs…
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Characterizing Endothelial Cell S1P Receptor Expression via Flow Cytometry
Introduction Much of the background information for this set of experiments can be found in my previous post. In short, endothelial cells mainly express two receptors to the sphingolipid S1P, S1PR1 and S1PR3. S1PR1 is characterized as a barrier enhancing…
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Wastewater Separation via Tangential Flow Filtration.
Thanks to Dan Ahmad for performing the filtrations. Introduction I want to see if we can improve the sample preparation method for the recovery of SARS-COV-2 from wastewater filtration using our nanomembrane technology. Currently, raw samples are ultracentrifuged against a…
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Lift-off Attempts for a Bone-Invasion Mimetic with Porous Roof using 100 nm NPN
This post is a continuation of the project found here: Fabricating 500 nm Channels in Silicon Wafers. The chief limitation of the previous device design was that the long narrow nanochannels were not effectively wetted. To combat this problem, we…
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