So this is the latest iteration of the thumb-pump device we were working on this summer. As you will see in the diagrams, the idea is to ultimately have saliva go through a filtration step, with a side channel leading…

Hi all – I finished my 10 months internship with Sartorius on 16th of July and since all my results are going to be the third and last aim of my Ph. D., I will be presenting my results in…

Background Previously we characterized HUVEC monolayer barrier via TEER in traditional transwell systems following polarized TNF-alpha exposure (Figure 1). One major shortcoming of this experimental set up for our study is it does not accurately reflect the permeability of HUVECs…

Introduction Much of the background information for this set of experiments can be found in my previous post. In short, endothelial cells mainly express two receptors to the sphingolipid S1P, S1PR1 and S1PR3. S1PR1 is characterized as a barrier enhancing…

Thanks to Dan Ahmad for performing the filtrations. Introduction I want to see if we can improve the sample preparation method for the recovery of SARS-COV-2 from wastewater filtration using our nanomembrane technology. Currently, raw samples are ultracentrifuged against a…

This post is a continuation of the project found here:  Fabricating 500 nm Channels in Silicon Wafers. The chief limitation of the previous device design was that the long narrow nanochannels were not effectively wetted. To combat this problem, we…

Fluorescent Streptavidin Coated Bead Capture on Biotin KODE Coated Surfaces After determining that KODE molecules can successfully coat our silicon pore membranes, we set out to determine if the coating could be functional and how stable it was. To test…

 Goal: Is to produce a membrane has pockets to capture the extracellular vesicles EV’s. Membrane structure: Figure 1 shows the membrane structure. The membrane will have two pores sides: upper and lower. The pores on top are bigger than the…

Wound healing experiments I have been isolating extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) and using them in wound healing experiments. For isolation, I follow the same protocol as outlined here. For these experiments, I used small EVs, which…

Goal In preparation for experimentation with hCMEC/D3, an immortalized BBB cells line, I characterized barrier formation by taking TEER. I want to find peak barrier formation to determine what day to run assays. Methods hCMEC/D3 were maintained and passaged in…