I recently repeated a chemical stability test of RTPed (5 min in Ar at 800C) samples from W613, which has the 30 nm pnc-Si layer. In this study I used DMEM +10% FBS as the culture media without cells. Previously…
Category: NRG
Rhodamine transport in transwells
I finished the first experiment to look at rhodamine transport across commercial transwell membranes. I was mainly interested if there would be enough transport into the basolateral chamber to measure the rhodamine with the spec. Experimental details: both chambers in…
Electrophoretic Cell Update
We were worried that Friday’s experiments were giving us a false positive results. If the electrophoresis breaks the membrane, then we’d expect to see the rhodamine rush through just like it was in our experiments. It’s too hard to view…
Posted in NRG
More on porosity vs. position
Last week I took a series of TEM images from all but one position on wafer 638. Below are the TEMs, histograms, and porosities vs. distance from center plots. “Xpos”, “xneg”, “ypos”, “yneg” indicate which axis the images were taken…
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Electrophoretic Cell
Because of Harold Smith’s project, Rachel and I have been working on various methods to get proteins through a pnc-Si membrane and onto a polymer membrane. The particular system that Dr. Smith wants to study is very sensitive to ambient…
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Discoloration – Cell Media
I’ve been learning the discoloration protocol from Karl and Anant and finally have some pictures of my own to show. We got a couple of new media (RPMI and DMEM/F12) last week in order to grow the cells I’m getting…
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SiO2 evaporation at RIT
Today Barrett and I processed SiO2 deposition at RIT. This process was actually delayed due to the cleanroom scrubber failure last week. We deposited 30nm SiO2 on both sides of three wafers, #335, 341, and 345. Earlier in March, I…
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"Separations" with pinholes
One question we recently had was what does it look like when we perform a separation experiment when a membrane has a pinhole. I selected two membranes off 621 that appeared to have pinholes on both slits. I applied 1:2…
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Pore Processing Comparison of W615
In order to support the idea that RTP treatment does not change membrane pore characteristics (as shown by Anant in the rhodamine diffusion study) I processed TEM samples that were not treated, RTPed in Ar, for 5 minutes at 800C,…
Update of W637
Dave delivered W637, a wafer made using the sample recipe as W504, to us today. This wafer has a high occurrence of single pinholes, with only 12 samples pinhole-free. I also noticed some odd features on the membranes, shown below.…
