Last week I tried to diffuse Cytochrome C through our membranes onto PVDF and PES membranes to see if we can detect protein adsorption on the other membranes. The trial illustrated that cytochrome c will diffuse through our membranes and…
Category: NRG
Polymer deposition updates
The 20nm deposited polymer film permeability is approximately 1e6 barrers, while bulk polyimide permeability is less then 100. So the experimental value is too high. There are two possibilities to explain it: First, the pores are partially open and 20…
Posted in NRG
Sepcon-Transwell hybrid devices
I’ve been working with the Corning transwell inserts and our SepCon units a bit recently. The addition of the Dremel last week has allowed better control while cutting the transwells, even without the right angle attachment (which will allow cutting…
DNA separations
Bill Bernhard’s group approached us about using pnc-Si membranes to separate DNA species by diffusion. Bill’s lab studies the effects of ionizing radiation (ex. X-rays, gamma rays, UV light) on DNA using electron paramagnetic resonance (EPR). There are three major…
Posted in NRG
Silanization and Membrane Chemical Stability
Due to our desire to improve membrane stability in cell culture, different methods of treatment have been mentioned to try, including carbonization and silanization. W606 was treated with UV/ozone at 150C for 20 minutes, followed by silanization performed by some…
Membrane Stability under various RTP Treatments: Retry
Previously W514 was tested with different RTP conditions (varying time at 800C, all in Ar); the results were difficult to interpret as the cells were confluent (in some cases it was hard to see membrane breakage). We therefore decided to…
HUVEC spreading on pncSi and glass
Following time lapse movies show the spreading of HUVECs on pncSi and glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~4 hrs. HUVEC spreading on pncSi HUVEC spreading on glass
Diffusion Separation with w612
This was something I set up a few days ago and finally got a chance to run the gel. I was curious to try this after the previous forced separations using brain extract. If you recall, the “separation” using the…
Posted in NRG
Smith Lab Diffusion Studies
Harold Smith at the Med Center proposed an experiment for Jess and I to try, in which we use our membranes to separate proteins onto a PES, PVDF (Polyvinylidene fluoride) or Nitrocellulose membrane, similar to a Western Blot of DNA…
Posted in NRG
Chemical Stability of 30nm Samples with RTP Treatment
Previously I ran a chemical stability study of samples from W613, which has a 30 nm thick pnc-Si layer. In that study complete failure of untreated samples was observed by 18 hours in cell culture, which was not a significant…
