I oxidized 2 intact chips from w639 using the following protocol: 10min with secondary generator, 15min with both primary and secondary generators. This protocol is slightly different than my normal protocol (10min 2nd, 5min 1st+2nd, 10min 2nd) because I messed…
Category: NRG
Bradford Assay to Quantify Protein Retention During Desalting
The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which…
Water transport in Electrophoretic Cell
I’ve tried to get some more data for the phenomenum that may be electro-osmosis in our electrophoretic cell. In the last post I looked at the rate of passage of 1x PBS in pinhole membranes from w621. After that I…
Posted in NRG
AFM of wafers
JP handed me a stack of wafers today, and I went over to the Krauss Lab and with the help of Jack Calcines got some AFM images. I didn’t have much time to play with, so I tried to get…
Posted in NRG
W648: Burst Pressure and the Return of Contamination?
We received W648 from the production team this week. Upon inspection of this wafer, is has a higher density of pinholes than we have been seeing lately (>3 pinholes/slit compared to 0 or 1/slit) and seems to show a return…
Karl's Air Permeability and Burst Pressure Raw Data
In this week’s meeting there was a request to have Maryna and I make our BP and AP data available online. While Maryna is at the Gordon Conference I thought I would upload what I have. The attached spreadsheet contains…
Conditioned DMEM – Discoloration
Motivated by Tom’s suggestion at lab meeting on Tuesday, I tried “conditioning” the DMEM+10%FBS before adding the chips. “Conditioning” just means that I allowed the DMEM+10%FBS to equilibrate in the incubator for an hour before adding the chips to test…
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AFM of polyimide covered wafers
There is AFM images that were taken in Krauss’s lab before I left. I wasn’t able to see open pores. There were samples from two wafers (213 and 398) with deposited polymer. On samples from first wafer, the polyimide deposited…
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Electro-osmosis and Electrophoresis Discoloration
Electro-osmosis: Yesterday I tried to study the electro-osmosis-like phenomenon that we were observing with the electrophoretic cell. All membranes again had pinholes. I set up the wells as before, but I would remove 50ul from the well which the negative…
Posted in NRG
SiO2-deposited discoloration
Last week, Lingyun and I deposited 30nm SiO2 onto both sides of wafers 335, 341 and 345. Unfortunately, these wafers weren’t in too good shape, so there are only a few intact slits without pinholes. I then took a few…
