Last week I ran cell culture growth experiments on RTPed (800C 5 minutes in Ar) samples from W567 (single slit 100um x 1000um). For this study I tracked discoloration w/cells, membrane stability w/cells, and the bead assay w/cells to see…
Category: NRG
Adsorption Quantification VI
Recently I tested track etched membranes and crystalline silicon (basically the backside of the membrane chips) following the sample buffer adsorption assay. This new data comprises the last two bars on the following chart. I’m surprised to see that crystalline…
Posted in NRG
Fibroblast spreading on the membrane
Time lapse movie of fibroblast spreading on the nanomembrane was captured over a period of ~ 8 hours. I was having some problem uploading the video here, thus have done so in the NRG knowledge page under the cell culture…
Cell Culture Applications
I’ve added a page on cell culture applications to the knowledge wiki. Watch this page and edit it if the topic is of interest to you. Remember the wikis provide a place to comment on the changes you make ……
Posted in NRG
Wafer Testing: W520, 558, 559
I recently received three pinhole-ridden wafers for cell-related experiments. All three have been RTPed at 800C for 5 minutes in argon, which is the typical procedure we have been using for cell culture. The bursts pressures for each wafer are…
Two-step anneal trial
Last week I mentioned that a two step anneal may help with the tears that are forming on the membranes when they are annealed post-etch. The recipe I used was a 10 C/s ramp to 600 C, hold 60 s,…
Posted in NRG
Success with Capillary Tube Inlet and Outlets
In order to test the sealing of the capillary tubing the PDMS I made a batch of PDMS with a recess in it (from a dime). Once this cured I used the UV Ozone process (@25C) to bond the PDMS…
Posted in NRG
Attempts at Closed Flow System
I wanted to update everyone on the recent attempts at the formation of the a closed flow system by bonding PDMS to our membrane chips, using capillary tubing for the inlet and outlets. Last week when we tried to preform…
Gel from Diffusion Chamber
Jess showed me how to use the diffusion chamber to separate proteins on the membranes. I ran a gel to see if any proteins appeared in the filtrate. The gel is posted below: The lanes are (from left): protein standard,…
