I recently received three pinhole-ridden wafers for cell-related experiments. All three have been RTPed at 800C for 5 minutes in argon, which is the typical procedure we have been using for cell culture. The bursts pressures for each wafer are…
Category: NRG
Two-step anneal trial
Last week I mentioned that a two step anneal may help with the tears that are forming on the membranes when they are annealed post-etch. The recipe I used was a 10 C/s ramp to 600 C, hold 60 s,…
Posted in NRG
Success with Capillary Tube Inlet and Outlets
In order to test the sealing of the capillary tubing the PDMS I made a batch of PDMS with a recess in it (from a dime). Once this cured I used the UV Ozone process (@25C) to bond the PDMS…
Posted in NRG
Attempts at Closed Flow System
I wanted to update everyone on the recent attempts at the formation of the a closed flow system by bonding PDMS to our membrane chips, using capillary tubing for the inlet and outlets. Last week when we tried to preform…
Gel from Diffusion Chamber
Jess showed me how to use the diffusion chamber to separate proteins on the membranes. I ran a gel to see if any proteins appeared in the filtrate. The gel is posted below: The lanes are (from left): protein standard,…
Desalting: Continued Work
In continuation of the desalting work, I ran three additional controls: a SepCon with no slits (negative control) and direct addition of 4M NaCl producing final dilutions of 1:400 and 1:600 (these are the experimental final dilutions I have been…
AJA film characterization
I used the spectroscopic ellipsometer to measure several wafers deposited using the AJA system. I verified film uniformity as well as film quality using index of refraction as the metric. The term “w/ Bias” refers to an applied RF power…
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Gold in Sodium Tetra Bromate
At Nanotech I met a professor from Geneseo, K. Yokoyama, who worked with gold in sol-gels. He found he was able to dissolved gold in sodium tetra bromate (Na2Br4O7) without it aggregating. I have attempted to dissolve gold in other…
Posted in NRG
Serial Dilution of Rhodamine
I was having trouble using the spectrometer to measure rhodamine concentration in the stir cell. I ran a standardized absorbency of the serial dilution of rhodamine. The curve is posted below. It appears to be linear. The molarity of rhodamine…
