Wafer#502 was coated with 10nm_Ti & 15nm_Au on Friday. I will pick out pinhole free membranes later today. I will also try to get TEM images at Karen’s lab (medical center). // The RIT clean room is closed this week.
Category: NRG
Adsorption Quantification I
UPDATE: Figure normalized by frontal surface area. This brings the glass data in line with pnc-Si (which is similar to my more recent fluorescence data). Here is my first try at putting together all the recent adsorption gels I’ve done.…
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BSA Adsorption Studies 2
In our previous adsorption study, all of the surfaces seemed to adsorb a similar amount of protein. We thought this might be due to ineffective washing. In this study I tried a better washing technique: Incubate polymer membranes, pnc-Si chips,…
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Fluorescence Adsorption Assays
Glass chips and pnc-Si membranes were incubated in 1mg/mL FITC labeled BSA for 30min. They were vigorously washed in a large beaker of PBS. Chips were placed on a slide and imaged with fluorescence scope. For this study, it seems…
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Production from week 5.12.2008
Wafers 525 & 527 have a 1000 Å dry thermal oxide mask with all three films on the frontside sputtered. Both these wafers had a high density of pinholes. The pores are also irregular in shape and seem to have…
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W502 and 504 Testing
W502 and 504 were tested for burst pressure and air permeability. Only one sample from W502 was tested for burst pressure (the other broke while setting up and I wanted to conserve samples) with a burst pressure of 4.46 psi.…
HUVEC Growth and associated pnc-Si discoloration
HUVEC (P8) growth was monitored over 5 days on different substrates (glass coverslips, tissue culture treated plastic and pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate on pncSi in…
Fibroblast Growth and Associated pnc-Si Discoloration
A second fibroblast (L1 3T3, P21) growth test was conducted on RTP treated samples from W510, tissue culture plastic (polystyrene), and glass cover slips. Fibroblasts were cultured in DMEM with media changed every 48 hours. Cells were imaged using the…
Production from week 5.5.2008
Last week’s production yielded two wafers (502 & 504) with defect-free membranes. 506 had a high pinhole density. These wafers have a TEOS mask and were annealed twice; the first anneals did not produce pores so they were annealed again…
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BSA adsorption comparisons
I set out to do an in depth study of adsorption to various surfaces using the sample buffer method. Midway through staining the multiple gels I noticed there was a problem. It looked as though glass and polystyrene adsorbed about…
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