Cody Soule (MS student) developed/applied a protocol to functionalize the surface of a silicon oxide membrane. The process selectively attaches PNIPAM to the surface of the membrane, maintaining the pore characteristics unchanged. PNIPAM undergoes a configuration transition when going from…

Adeel and I summarized our key takeaways from the BMES in this post. I personally tried to attend organ on a chip talks and posters, which included a micro- or nano-porous membrane or a micro/nano-structured co-culture system. I tried to…

MATLAB updated the way that it creates GUI applications back in 2015, and it is increasingly becoming more functional. Over the years I have been here, we have had more than a few requests to use our pore processing software.…

Hello everyone, Aslan and I attended talks regarding EVs enrichment which can be divided into two broad categories depending on their ability to recover and reuse the EVS or not. There is a recent review called “New technologies for analysis…

Hi everyone, this is a short post quickly detailing some interesting quirks of different wafers as their respective chips are subjected to a control experiment (1x PBS, Qs = 30 ul/min, Qu= 10% Qs every 45 minutes). First, lets look…

BMES – Atlanta 10/17/18-10/20/18 F-actin Alignment Quantification Using HSB Color Coded Maps ImageJ Plugin: OrientationJ Purpose: Quantification of F-actin alignment using ICC. HSB map provides a visual representation of fiber alignment with color codes related to hue:local orientation, saturation:coherency, brightness:fiber…

With the installation of the new STED (STimulated Emission Depletion) system in the medical center, I have finally been able to work on imaging individual exosomes (that we know are exosomes because they are labeled for exosomal markers) that have…

Summary I need to replace the function of my breath to get water into the femtoliter sized cavity of the monolithic device. I achieve this using a small stream of ethanol vapor as a precursor, and then replace the ethanol…

Introduction In severe sepsis, a primary infection gives way to a maladaptive immune response leading to wide spread edema and multiple organ dysfunction. The initial infection can occur in many peripheral tissues throughout the body. The pathogen associated molecular patterns…

Alec and Elysia’s study on the migration of infectious bugs through microporous silicon nitride has been useful, however, the size range of the pores is not sufficient to halt the migration. To create smaller pore sizes, I infilled the chips…