Category: NRG

PKH Paper Final Analysis and Figures

Hello Everyone, This is an update on all my experiments and analysis on PKH paper. As i had a post before about PKH nanoparticles and PKH-Exosome fusion after labelling, we’ve been working on analysis and finishing figures for this paper.…

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Cell culture shear test with and without debubbler

What happens to cells when they are exposed to a series of bubbles with and without a dubbler? The purpose of this experiment was to prove the hypothesis that bubbles damage cells, and the dubbler prevents cells from experiencing bubble…

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Rotation Summary: A study on neutrophil migration through collagen coated nanoporous membrane

This post serves as a summary of the work done during my rotation in the McGrath lab this summer. Over the course of six weeks, I shadowed projects covered in the lab along with the specializations of microfluidic devices and…

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Update: Critical Flux Experiments, Trial and Error

Hi everyone, in this update I’m going to go over some of the highlights from my experiments over the last month. After going through several trials that ultimately ended in failure, we moved over to testing long term performance of…

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Improving Particle Capture Predictions with COMSOL

In Episode 3 of this series, I have begun working towards improving my COMSOL modeling for particle capture. In addition to running simulations, I need to run experiments to try and correlate real world solutions with simulated results. One of…

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Preliminary Fabrication of a Tenting Well Oxide with a Buried Electrode

We want to create a structure that can more easily control the electric field inside of the Monolith cavities, by adding an electrode specific to each well. My solution is to create a hybrid film stack, replacing some of the…

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Cancer Cell Migration and Wound Healing

A few weeks ago, Henry and I ran a migration/wound healing experiment using two cancer cell types: T24 (bladder cancer) and MDA-MB-231 (breast cancer). MDA cells are highly migratory, while T24 cells are less so. We wanted to test if…

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Protein Binding by Suspended Graphene Oxide

Experiments were conducted to determine how effectively protein is bound by suspended graphene oxide (GO) particles. This is important for our application because we hope to use GO as a membrane to bind toxins present in blood. BSA (bovine serum…

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Progress on Wetting Monolith Chips

Kyle has made some progress using the monolith chips, however, the wetting of these chips is difficult and makes the monoliths unattractive to use. I have attempted to create a more replicable wetting protocol. We’ve heard over the summer that…

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Double Slot: Single-Pass Dialysis Devices Update

This post is meant to conclude the work that I have completed this summer with the double slot single pass devices; recently I have conducted additional experiments and collected ultrafiltration data. In my previous post, “Single Slot Inverted-Chip and Double…

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