Introduction In response to recent requests by our reviewers, I am looking to address two main concerns: 1) How does a collagen gel backing affect leukocyte transmigration, and 2) How does shear influence leukocyte transmigration. While we believe these areas…

As a proof of principal experiment, we wanted to test if Kilean and I could extract RNA from exosomes following their capture on 1298 membranes.  As noted in Figure 1, we first used ultrafiltration to capture exosomes on the nanoporous…

Optimization of the PLL-g-PEG coating on SiO2 substrate: The first goal was developing an efficient method for PLL-g-PEG coating which provides a high density of PLL-g-PEG grafting on SiO2 substrate and effectively prevent cell adhesion. To achieve this goal, different…

Okay, so after navigating this weird new method for posting, I have a semi interesting post today. I have been working on the experimental validation of my COMSOL predicted capture results and have finally completed the first round of those…

So as most of you know by now, I have been working on developing microfluidic systems to study the leukocyte-endothelial interactions in vitro. I have developed systems that can facilitate the basic biological assays involving neutrophil migration and live-imaging. In…

A collaborator of mine is interested in arranging quantum dots on a surface to demonstrate something called super irradiance. All of the sub-resolution particles could emit as one object (think laser) if the conditions are just so. Ideally, a bunch…

Cody Soule (MS student) developed/applied a protocol to functionalize the surface of a silicon oxide membrane. The process selectively attaches PNIPAM to the surface of the membrane, maintaining the pore characteristics unchanged. PNIPAM undergoes a configuration transition when going from…

Adeel and I summarized our key takeaways from the BMES in this post. I personally tried to attend organ on a chip talks and posters, which included a micro- or nano-porous membrane or a micro/nano-structured co-culture system. I tried to…

MATLAB updated the way that it creates GUI applications back in 2015, and it is increasingly becoming more functional. Over the years I have been here, we have had more than a few requests to use our pore processing software.…

Hello everyone, Aslan and I attended talks regarding EVs enrichment which can be divided into two broad categories depending on their ability to recover and reuse the EVS or not. There is a recent review called “New technologies for analysis…