This post is a recap on the nanosphere lithography based membrane patterning process. The results shown here are from a non-optimized run of the process. The image below shows an overview of the fabrication process used.   Below you can…

In previous experiments attempting to culture ADSC on non-porous and porous (0.5 µm, 3.0 µm) SiO2 membranes, we were having difficulty getting the ADSC to attach to the membranes. A majority of the ADSC did not appear well-spread. While in…

Hi all, Recently we finished drafting the manuscript titled “Porous substrates promote early endothelial migration at the expense of fibronectin fibrillogenesis.” This manuscript will feature the cover illustrated by Brad (our new medical illustrator in the Gaborski Lab):   Long…

We are happy to be back this summer. Anthony has been working on setting up a system that mimics Kilean’s dialysis devices. Our approach is different in two important ways. First, we are using PETLs, instead of PDMS/glass.  For those…

Some background and a quick summation since I have done a great job at avoiding posting. My undergrad background before the lab Chemistry, physics, math (minor) and a random psychology minor biology I questionably knew what a cell was Overarching…

When we are trying to optimize the capture of exosomes in the tangential flow system, it is important to understand how particles are behaving in the flow. Initially, we tried to do this last summer by looking into the particle…

Wafer 1148 was produced roughly around the same time that the wafer I used to make my oldest MgF2 material (wafer 1085) was produced. We know that some of the NPN material from that time was not etched all the…

Introduction As a continuation of my rotation project and a beginning to my PhD work in the McGrath lab, I wanted to finalize my shear stress calculations within our blood vessel microfluidic device. All previous shear stress calculations were purely…

Just a quick update on my status: I got the NPN pre-filters from Greg in Montreal and finished testing 15 of them last week, so this week I will start doing additional experiments. I plan to explore the size separation…

This is my (Jim) summary of the results thus far for NPN vs NPN-O and Front Side/Back side comparisons. More to come … *************************************************************************************************************   As thesis indicated, the thicker membrane is, the larger average pore size will be. From…