In trying to determine the best conditions for capturing exosomes, one of the important questions that we have is what produces the case where exosomes are caught directly in the pores, not on top, but flush with the surface. This…

As the title states, I partially etched some different material (Wafer 2238, 100 nm NPN) to create nanofeatures with blown out holes. With a thicker membrane, we can blow out the pores more (etch longer) without compromising the strength of…

Matlab Generated Endothelial Cell Alignment Distribution Top and Bottom Gel No Gel Bottom Gel Run 1 Run 2 Statistical Analysis The next step in this data analysis is to perform some form of statistical analysis. Comparing real alignment data to a set…

This post is a continuation of the work in Partial Etch RIE Blows out NPN Pores and MgF2 Nanomembrane Fabrication on partially etched NPN substrates. We believe the underlying NPN template influences the shape of our SERS active nanostructures and therefore the…

[17-01-13] Committee Meeting

This post is a brief update on the previous post of HUVEC migration on 0.5 μm porous substrates.   Brief Recap We compared cell migration on nonporous (np), 0.5 μm low porosity, and 0.5 μm high porosity SiO2 membranes. Low and…

Here are the first experiments I have performed with the SepCon slots, under the guidance of Jamie and Kilean. The goal of these experiments is to prove that goat anti-human beads (6.8 um diameter) can be combined with a low…

As a follow up to my previous post, I would like to present some preliminary data on the subject of endothelial cell alignment under shear stress with variable substrate stiffness. The flow set-up (Figure 1) allows for steady flow across…

I’ve been culturing RPE cells over here in Rochester for my MgF2 fabrication paper, in which the goal is to make a permeable Raman compatible cell substrate. My previous work had shown polarization of RPE cells (via-ZO-1 staining) on nanoporous…

As you may already know, we are writing a review article for the Electrophoresis journal on nanofabrication methods. This post is to request your feedback on the figures included in the manuscript. The main objective of figure 1 is to…