We have an STTR grant funding us to revise the Sepcon plastic housing to enable us to do reverse centrifugation easier. As outlined in this previous post, we’re trying to remove unbound IgG from a solution of IgG-antibody conjugates using…

Below are drafts of all the figures I am planning on incorporating into my upcoming model paper. I believe I am done with data collection at this point. Data in figures not marked “NEW” probably needs to be updated. Note that all model…

The process to create free standing films of MgF2 requires the film stack to survive a few different kinds of stresses. First, there is thermal stress from the mismatched coefficients of expansion (MgF2 expands about 5-10x more than silicon/silicon nitride),…

This update covers my efforts since our last skype meeting to show DNA size separation in a filter, as well as some very interesting TEM images of ALD-coated NPN and associated controls. Here is an inventory of my current chips:…

While I have been making nanoporous MgF2 for a while, it has become obvious that the strength of the material is handicapping our ability to generate data; as the membranes rupture we lose weeks of cell culture work. While we…

Just a quick update this time – since my last post I have been doing control experiments to demonstrate the effect of the filters. For the controls I have been using the chips without the nitride cap, since I am…

For last 1.5 years, I have been working on developing a COMSOL model to understand how the geometry of pncSi chips affects the TEER values when cells are growing on them. I addressed this issues in some of my previous…

Since my last update things have been going quite smoothly. Most pores I make are able to pass 3 or 4 samples before clogging, and I have filled in the various graphs quite nicely. I still would like to flesh…

Hi all, just a quick posting to update you and deposit the current SOP and video instructions for PETLs. Nick has been working this summer on culturing cells on PETLs; its been slow, but we are finding that cells (HEKs)…

With changing my channel height to attempt to lower the Peclet number, one important factor that is affected is the transmembrane pressure. Decreasing the channel height will significantly increase the transmembrane pressure, which may stress the membrane and cause it…