While I have been making nanoporous MgF2 for a while, it has become obvious that the strength of the material is handicapping our ability to generate data; as the membranes rupture we lose weeks of cell culture work. While we…
Category: NRG
Control Experiments for NPN Translocation Project
Just a quick update this time – since my last post I have been doing control experiments to demonstrate the effect of the filters. For the controls I have been using the chips without the nitride cap, since I am…
Posted in NRG
Final COMSOL Analysis to Interpret TEER values from pncSi Transwells
For last 1.5 years, I have been working on developing a COMSOL model to understand how the geometry of pncSi chips affects the TEER values when cells are growing on them. I addressed this issues in some of my previous…
Posted in NRG
Update on entropy tricks with NPN
Since my last update things have been going quite smoothly. Most pores I make are able to pass 3 or 4 samples before clogging, and I have filled in the various graphs quite nicely. I still would like to flesh…
Posted in NRG
FISHER team and PETL instructions
Hi all, just a quick posting to update you and deposit the current SOP and video instructions for PETLs. Nick has been working this summer on culturing cells on PETLs; its been slow, but we are finding that cells (HEKs)…
Posted in NRG
Peclet Number Analysis Update
With changing my channel height to attempt to lower the Peclet number, one important factor that is affected is the transmembrane pressure. Decreasing the channel height will significantly increase the transmembrane pressure, which may stress the membrane and cause it…
Posted in NRG
Optimizing Tangential Flow Exosome Capture: A Peclet Number Analysis
Hi all, So this is slightly different from my previous post, but is perhaps much more important for the work that I am doing. This analysis is important because it allows us to determine the appropriate conditions at which…
Posted in NRG
A Clampable Tangential Flow Device for SEM Imaging
Hey all, So, I’ve been trying to replicate the exosome experiments that I did back during my rotation to varied success. I have had trouble with the plasma that we had frozen with precipitates that would not resuspend upon thawing.…
Posted in NRG
NPN Liftoff Update – 7/7/16
The NSF STTR phase I project officially ended last week, and we are writing the final report and preparing to submit a Phase II proposal. I will attempt here to summarize the program achievements and list some of the persisting…
Posted in NRG
