As a step to identifying the particles that I am capturing in my systems, I am attempting to do an immunogold labeling with a CD 63 primary antibody and a 10 nm gold conjugated secondary. It is possible to run…

The following contain updates from a group of Swiss collaborators working with NPN for a BBB mimetic. The movies are important because they demonstrate T-cell/endothelial interaction including transmigration and because they show how using vacuum pressure can rapidly cause vacuoles…

Here is a PDF of the presentation I plan to give at BMES this year. The presentation is first an overview of our SiO2 membranes for cell barrier and co-culture models and then a brief summary of our cell-substrate and…

The week before last, I finally switched back to my exosome experiments with some hope of repeating my January successes with my new clampable format and two pump system. After failing for several hours (and breaking 7 membranes), I was…

The title really says it all. I have been attempting to make a better cell culture substrate with the Magnesium Fluoride Films. Previous posts have shown the overall structure of the film, and now I am trying to quantify the…

Over the summer, I have been working on a project with Rick Waugh that focuses on separating white blood cells from red blood cells. The goal is to be able to take a lancet droplet of whole blood and perform…

We have an STTR grant funding us to revise the Sepcon plastic housing to enable us to do reverse centrifugation easier. As outlined in this previous post, we’re trying to remove unbound IgG from a solution of IgG-antibody conjugates using…

Below are drafts of all the figures I am planning on incorporating into my upcoming model paper. I believe I am done with data collection at this point. Data in figures not marked “NEW” probably needs to be updated. Note that all model…

The process to create free standing films of MgF2 requires the film stack to survive a few different kinds of stresses. First, there is thermal stress from the mismatched coefficients of expansion (MgF2 expands about 5-10x more than silicon/silicon nitride),…

This update covers my efforts since our last skype meeting to show DNA size separation in a filter, as well as some very interesting TEM images of ALD-coated NPN and associated controls. Here is an inventory of my current chips:…