The following images are from ARPE-19 Cells cultured for 3 Weeks over here in Nottingham (thanks for help from Emilia Moradi, Victoria Ciampani, and Kevin Webb). MgF2 nanomembrane chips were positioned into a transwell insert and sealed using PDMS, a biocompatible polymer, creating…

Overview – The purpose of this study was to perform in vitro hemocompatibility testing (in accordance with ISO standards) on material substrates that could come in contact with blood when incorporated into the final device design of a portable hemodialysis…

Introduction: At the last meeting, I introduced the new stirred cell device acquired in Tom’s lab (here). I have expanded the study to observe changes in flux and concentration for each of the membranes used. Experiments: Each of the experiments was…

In my earlier posts, as well as in Josh’s NPN paper and Tom’s ACS nano paper, we’ve talked a lot about the resolutions of nanoparticle separations. What we mean by it is how well the membrane is able to discriminate between…

Introduction: The lab purchased an Amicon 8010 Stirred Cell which has a 25mm diameter for membranes and holds 10mL of solution. The device works by placing the solution into the cell, pressurizing the system at 8-10 psi, and creates stirring via…

Since the new year there have been difficulties with charging the glass plate and the charge not being enough to capture the membrane.  Synthetic silk was tested to see if the glass charged better but no difference was seen between…

Objective Develop an easily mainpulated reproducible vasculogenesis assay using hydrogels laden with endothelial cells (‘bio-ink’) patterned using the technique of extrusion bioprinting Bio-inks Develop a bio-ink with the following characteristics Cell viable: bio-ink must sustain cell life, allowing cells to manipulate and move…

As part of my work over at the University of Nottingham, I’ve been using iBidi devices to grow cells. Eventually, I will use their product to do Raman studies of cells grown on my MgF2 substrates. I’ve been making some…

Hey all! In this post, I’d like to present some of my final data on the exosome isolation project and cover some data that was generated in response to questions asked during our previous discussion. To begin with, Tom asked…