I am doing a few more membrane transfers and trying to troubleshoot my delamination problems, and I wanted to run my protocol by you guys before I mess up any more chips. I was able to get about 3/7 transfers…

Since the control experiments seem to be going OK I decided to try again with one of the NPN-tented structures that Greg made originally. Since piranha cleaning seemed to be making a difference I wanted to pre-clean the chip, but…

Phase II submission August 1, 2016 or January 30, 2017 UR Tasks – Biocompatibility by Feb. 1 2016 Channel design optimization by November 2015

The idea here is to use nano membranes that have been integrated in a chip with the intention to mix otherwise laminar flow streams.   It was suggested at one of the meetings that I should superimpose the streams, then…

The following post is the abstract that I recently submitted for the BMES Annual Conference. Introduction: Permeable membranes provide a physiologically relevant cell culture substrate for in vitro study of barrier cell types, such as endothelial cells, which assume a…

To continue our spreading and proliferation experiments on various topographical features and stiffness, I repeated the HUVEC experiments I had done before due to a possible artificial bias that we think is a result of high seeding.  To enusre accuracy,…

In order to investigate the breakage of nanoporous nitride hemodialysis chips during rat dialysis, we are investigating the pressures in the system. We will investigate normal operating pressures as well as potential impulse pressures from handling or valve operation. To…

Conventional tissue culture substrates do not accurately emulate in vivo surface conditions. This project involves the development and characterization of a polydimethylsiloxane (PDMS) substrate for cell culture. PDMS is an important biomaterial due to its optical transparency, biocompatibility, and variable elastic…

Just wanted to show the detail of PETL channels.  A couple of interesting things:  The adhesive layer(s) are pretty thick, and look pretty smooth after heat curation.  Also, the channels are slightly beveled, probably due to craft cutter blade.

Utilizing a cation-exchange membrane like Nafion makes it possible to electrically induce the concentration difference across the membrane. The resulting osmosis of water augments the overall flowrate in addition to the electroosmosis. The thinner the membrane, the stronger the osmotic…