This paper (SiN paper) by Ulrich Rant et. Al. details several experiments conducted with a single pore drilled through a SiN membrane via TEM. They measure the relationship of pH vs. zetapotential for both a model protein (avidin) and their…

Final-Program-MMD-2014.prog_ June 19 Random Notes •    Blood Stasis is the main cause of thrombosis •    23 French can access the venous system •    3 French per mm •    Extracorporal Membrane Oxygenator used in the treatment of Cardiogenic Shock ◦    Use…

Glavan et al 2013 Lab on a Chip This paper from the Whitesides group details an interesting way to fabricate paper-based microfluidics. They treat ordinary paper with a fluorinated silanization process to render it hydrophobic, and  use a Silhouette cutter…

This paper was found when looking for details on quantifying cell spreading and adhesion. The paper discusses how the group has made mesoporous silicon wafers and their success with growing human umbilical vein endothelial cells on the wafers without additional…

Last summer, I attempted to modify the surface of SU-8. The purpose of the experiment was to covalently bond poly(ethylene glycol) (PEG)  to SU-8, which would be useful when the SU-8 is used as a scaffold on the membranes. The…

To determine whether cell proliferation is a function of membrane porosity and/or pore size, human umbilical vein endothelial cells (HUVECs) were cultured on various surfaces.  The cells were seeded on low and high porosity membranes of 0.5 um and 3.0…

Overview: This experiment was conducted to compare the proliferation of adipose derived stem cells (ADSCs) when grown in different medias. Two medias were used and each had different growth factors and serums. Only 0.5um low porosity membranes were used for this…

This week, I tried to make a large number of MgF2 nanomembranes for my collaboration with Kevin Webb.   I wanted to keep the evaporated surface as pristine as possible, so instead of using the Kapton tape to attach the chips…

The blue line reflects measurements taken with my zetapotential setup. Each data point is the average of three independent measurements of zetapotential (a new 3-slot SiN wafer 1024 chip for each measurement) and the linear fit for the pressure vs.…

Overview: This experiment was conducted to determine the difference in human umbilical vein endothelial cell (HUVEC) spreading on different membranes. The cells were grown on the membranes for a specified length of time and then stained and imaged to determine the…