Last summer, I attempted to modify the surface of SU-8. The purpose of the experiment was to covalently bond poly(ethylene glycol) (PEG)  to SU-8, which would be useful when the SU-8 is used as a scaffold on the membranes. The…

To determine whether cell proliferation is a function of membrane porosity and/or pore size, human umbilical vein endothelial cells (HUVECs) were cultured on various surfaces.  The cells were seeded on low and high porosity membranes of 0.5 um and 3.0…

Overview: This experiment was conducted to compare the proliferation of adipose derived stem cells (ADSCs) when grown in different medias. Two medias were used and each had different growth factors and serums. Only 0.5um low porosity membranes were used for this…

This week, I tried to make a large number of MgF2 nanomembranes for my collaboration with Kevin Webb.   I wanted to keep the evaporated surface as pristine as possible, so instead of using the Kapton tape to attach the chips…

The blue line reflects measurements taken with my zetapotential setup. Each data point is the average of three independent measurements of zetapotential (a new 3-slot SiN wafer 1024 chip for each measurement) and the linear fit for the pressure vs.…

Overview: This experiment was conducted to determine the difference in human umbilical vein endothelial cell (HUVEC) spreading on different membranes. The cells were grown on the membranes for a specified length of time and then stained and imaged to determine the…

HI everyone, We continued working with Henry and Greg, gaining expertise on building chips and print-cutting channels.  Right now we have a bunch of chips made, some have been tested, and we have completed one of our objectives : To…

Over the weekend, I resubmitted our adult Adipose-Derived Stem Cell (ADSC) paper titled: Membranes Promote Endothelial Differentiation of Adipose-Derived Stem Cells and Perivascular Interactions There were three main findings: 1.) ADSCs can be induced to express CD31 (PECAM-1) like endothelial…

I went over to RIT to work with JP on measuring the deflection of NPN and pnc-Si membranes as a function of pressure.  JP and Dave Fang did this work years ago, and we want to pick it up again…

This past year I have been collecting burst pressure data on pnc-Si and NPN. The data for 30nm pnc-Si came from my previous post, “Wafer 1059: Burst Pressure Analysis”, which also includes a wafer map. This wafer had 7% porosity and…