With the help of Henry and Greg, the Fisher team has been busy learning the basics.  John and Ryan have learned some PDMS microfluidics-making and will have their first chips ready on Tuesday.  We have also been playing with the…

I’ve been going to considerable lengths to try and get reproducible zetapotential measurements from my streaming potential device. Zetapotential measurements are the closest we can experimentally get to measuring the surface charge of a membrane, and are important for three…

We are carrying out filtrations using wafer 1085.  We are testing the total volume of filtrate that passes in a given time.  We expect the total filtrate volume to decrease as concentration increases due to more immediate clogging.  Ultimately, we…

I am trying to create ITO electrodes for my microfluidic device for TEER measurement system. In my previous posts, I have shown that silver/silver chloride electrode, which is most commonly used in most commercial TEER systems, had killed the cells…

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This week I’ve added male connectors to my device. Originally, I had female-female tubing connections to the device, which were very prone to leaking around the insertion ports. Tejas suggested that I try printing out the male connectors to combat…

The overarching goal of this collaboration is to expose students and faculty at primarily-undegraduate institutions to the technology & expertise available at the University of Rochester.  We would like to make this kind of experience a recurring theme at URNano,…

The goal of the experiment was to use a fluorescent dye to label live cells in order to measure proliferation over several days.  To avoid cell toxicity, the intensity of the fluorescence was reduced to ~1.5%.  Human umbilical vein endothelial…

Hello NRG! Thanks to Jim McGrath and NSF, St. John Fisher College students and myself will have the opportunity to conduct research at URNano and the McGrath Lab this summer.  I am an Assistant Professor at Fisher in the Biology…

For a while now I’ve been running diffusion experiments using bacterial alkaline phosphatase (bAP) as my solute of interest in a solution of 50mM tris buffered saline (TBS) and 20 ug/mL BSA. Initially, the BSA was included as a way…