Category: NRG

Rat Dialysis Device Animations

Here’s a few sketchup animations I made for Dean’s device, based on his PPG presentation last week:      

Posted in NRG

RIE Etching Si3N4 to Make Freestanding MgF2 Nanomembranes

Poor English Paper used as a starting point: Silicon Nitride Etch Rate paper This is a continuation of the work I’ve done, attempting to make freestanding MgF2 nanomembranes (theory and deposition) using a Silicon Nitride nanoporous substrate. I originally  wanted to

Posted in NRG

300nm Thick SiO2 Membrane Soak Test

In this experiment, I soaked membranes (from either wafer 1064 [150W deposition power] or wafer 1065 [100W deposition power]) in different fluids that are standard use in cell culture applications, or which provide a similar situation as could be found

Posted in NRG

Zetapotential of untreated pnc-Si varies with pH

EDIT – 2/10/14 – Adding 1 mM phosphate buffer fixes the problems outlined in this post. I’ve been having some trouble taking consistent pH measurements with the new pH probe. Prior to Thanksgiving I made a spectrum of 10 mM

Posted in NRG

HUVEC Growth on Various Substrates

In evaluating the usefulness of our membranes for cell culture, it is important to understand if and how cell growth is affected by the surface.  The desire is that they be consistent with standard culture substrates (i.e. Tissue Culture Plastic

Posted in NRG

MgF2 E-beam Deposition

I am attempting to fabricate a free standing, porous, MgF2 nanomembrane for Raman spectroscopy applications (see my previous post for background). To that end, I’ve used the Lesker p75 evaporator in the basement to deposit ~100nm of MgF2 on one

Posted in NRG

MicroPorous Oxide Membrane Extraction

I recently popped out membranes from wafers 1064 and 1065 for our lab. I only started with about half a wafer, but these are the numbers from the extraction: Wafer ID # of In-Tact Extracted Membranes : High/Low Porosity #

Posted in NRG

FLAG Diffusion Experiment Progress Update

For context, see my previous post. Early on in the process of designing an experiment to confirm the usefulness of a CytoVu assembly for use as an assay, it was decided that the best solution for measuring the extremely low

Posted in NRG

Zetapotential Measurements of pnc-Si, SiN, and Stripped SiN Membranes

My streaming potential device is now working consistently. It turns out that the problem was the concentrations of salt that I was using. The Debye Length (the distance over which charge-charge interactions are important in an electrolyte) is given as

Posted in NRG

Endothelial Vacuoles Induced by Flow

In working on a long over due paper by Barrett on endothelial response to high permeability material … Key figs … Figure 2 Figure 4 Figure 5 And one post from Nakul. I came across this highly relevant paper Pedrigi(Overby)EER11

Posted in NRG
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